期刊
IMMUNITY
卷 54, 期 7, 页码 1527-+出版社
CELL PRESS
DOI: 10.1016/j.immuni.2021.04.022
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类别
资金
- National Institute of Neurological Disorders and Stroke (NINDS) [NS094573]
- University of Pittsburgh
- U.S. Department of Veterans Affairs (VA) grant [I01 BX003651]
- VA Senior Research Career Scientist Award
- Duquesne University
- NIH
The study found that after experimental stroke, Treg cells infiltrate the brain in mice and promote white matter repair and functional recovery. Treg cells enhance microglial reparative activity, promoting oligodendrogenesis and white matter repair. Increasing Treg cell numbers can improve white matter integrity and rescue neurological functions in the long term.
The precise mechanisms underlying the beneficial effects of regulatory T (Treg) cells on long-term tissue repair remain elusive. Here, using single-cell RNA sequencing and flow cytometry, we found that Treg cells infiltrated the brain 1 to 5 weeks after experimental stroke in mice. Selective depletion of Treg cells diminished oligodendrogenesis, white matter repair, and functional recovery after stroke. Transcriptomic analyses revealed potent immunomodulatory effects of brain-infiltrating Treg cells on other immune cells, including monocyte-lineage cells. Microglia depletion, but not T cell lymphopenia, mitigated the beneficial effects of transferred Treg cells on white matter regeneration. Mechanistically, Treg cell-derived osteopontin acted through integrin receptors on microglia to enhance microglial reparative activity, consequently promoting oligodendrogenesis and white matter repair. Increasing Treg cell numbers by delivering IL-2:IL-2 antibody complexes after stroke improved white matter integrity and rescued neurological functions over the long term. These findings reveal Treg cells as a neurorestorative target for stroke recovery.
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