期刊
IET NANOBIOTECHNOLOGY
卷 15, 期 2, 页码 149-156出版社
WILEY
DOI: 10.1049/nbt2.12031
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资金
- Sri Ramaswamy Memorial (SRM) Institute of Science and Technology, India
In this study, mesoporous silica microspheres were used as a support for the immobilization of inulinase from Aspergillus brasiliensis MTCC 1344 through cross-linking. The optimized conditions led to a high immobilization yield of inulinase, resulting in efficient hydrolysis of inulin to produce high-fructose syrup at a high temperature.
The authors used mesoporous silica microspheres as a support for the immobilization of inulinase from Aspergillus brasiliensis MTCC 1344 by the process of cross-linking. Under optimized operating conditions of pH 6.0, particle/enzyme ratio of 2.0:1.0 and glutaraldehyde concentration of 7 mM, a maximum immobilization yield of 90.7% was obtained after a cross-linking time of 12.25 h. Subsequently, the cross-linked inulinase was utilized for the hydrolysis of 5% inulin, and a maximum fructose concentration of 31.7 g/L was achieved under the optimum conditions of pH 6.0 and temperature 60 degrees C in 3 h. Furthermore, on performing reusability studies during inulin hydrolysis, it was observed that the immobilized inulinase could be reused up to 10 subsequent cycles of hydrolysis, thus providing a facile and commercially attractive process of high-fructose syrup production.
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