期刊
HISTOCHEMISTRY AND CELL BIOLOGY
卷 155, 期 6, 页码 699-718出版社
SPRINGER
DOI: 10.1007/s00418-021-01978-x
关键词
Organic dust; Mitoapocynin; Ethyl pyruvate; Mitochondrial dysfunction; Mitochondrial DNA
资金
- Iowa State University
- CDC-NIOSH (Centers for Disease Control and Prevention-The National Institute for Occupational Safety and Health) [5 U54 OH007548]
- National Institutes of Health [ES026892, ES027245, NS100090]
Exposure to organic dust rich in microbial pathogen-associated molecular patterns can induce lung inflammation and mitochondrial stress, potentially contributing to airway diseases. The study showed significant changes in cellular and organelle morphology, as well as the induction of mitophagy, upon organic dust extract exposure. Mitochondrial dysfunction and stress may be partially ameliorated by treatments with ethyl pyruvate or mitoapocynin.
Exposure to airborne organic dust (OD), rich in microbial pathogen-associated molecular patterns (PAMPs), is shown to induce lung inflammation. A common manifestation in lung inflammation is altered mitochondrial structure and bioenergetics that regulate mitochondrial ROS (mROS) and feed a vicious cycle of mitochondrial dysfunction. The role of mitochondrial dysfunction in other airway diseases is well known. However, whether OD exposure induces mitochondrial dysfunction remains elusive. Therefore, we tested a hypothesis that organic dust extract (ODE) exposure induces mitochondrial stress using a human monocytic cell line (THP1). We examined whether co-exposure to ethyl pyruvate (EP) or mitoapocynin (MA) could rescue ODE exposure induced mitochondrial changes. Transmission electron micrographs showed significant differences in cellular and organelle morphology upon ODE exposure. ODE exposure with and without EP co-treatment increased the mtDNA leakage into the cytosol. Next, ODE exposure increased PINK1, Parkin, cytoplasmic cytochrome c levels, and reduced mitochondrial mass and cell viability, indicating mitophagy. MA treatment was partially protective by decreasing Parkin expression, mtDNA and cytochrome c release and increasing cell viability.
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