期刊
HEPATOLOGY
卷 74, 期 1, 页码 379-396出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1002/hep.31607
关键词
-
资金
- National Natural Science foundation of China [31760258, 81673014]
- Program for New Century Excellent Talents in University, Ministry of Education of China [NCET-12-0661]
- Program for High-Level Innovative Talents in Guizhou Province [QKH-RC-2016-4031]
- Program for Excellent Young Talents of Zunyi Medical University [15ZY-001]
- Zunyi Science and Technology Bureau [ZSKH-SZ-2016-38]
- Zunyi Medical University [ZSKH-SZ-2016-38]
- Outstanding Academic Leaders Plan of Shanghai Municipal Science and Technology Committee [16XD1403100]
- National Key RD Plan [2017YFA0104600]
miR-7 plays a crucial role in autoimmune hepatitis, negatively regulating the activation and function of CD4(+) T cells to impact the pathogenesis of immune-mediated liver diseases.
Background and Aims Increasing evidence in recent years has suggested that microRNA-7 (miR-7) is an important gene implicated in the development of various diseases including HCC. However, the role of miR-7 in autoimmune hepatitis (AIH) is unknown. Approach and Results Herein, we showed that miR-7 deficiency led to exacerbated pathology in Concanavalin-A-induced murine acute autoimmune liver injury (ALI) model, accompanied by hyperactivation state of CD4(+) T cells. Depletion of CD4(+) T cells reduced the effect of miR-7 deficiency on the pathology of ALI. Interestingly, miR-7 deficiency elevated CD4(+) T-cell activation, proliferation, and cytokine production in vitro. Adoptive cell transfer experiments showed that miR-7(def) CD4(+) T cells could exacerbate the pathology of ALI. Further analysis showed that miR-7 expression was up-regulated in activated CD4(+) T cells. Importantly, the transcription of pre-miR-7b, a major resource of mature miR-7 in CD4(+) T cells, was dominantly dependent on transcription factor CCAAT enhancer binding protein alpha (C/EBP alpha), which binds to the core promoter region of the miR-7b gene. Global gene analysis showed that mitogen-activated protein kinase 4 (MAPK4) is a target of miR-7 in CD4(+) T cells. Finally, the loss of MAPK4 could ameliorate the activation state of CD4(+) T cells with or without miR-7 deficiency. Our studies document the important role of miR-7 in the setting of AIH induced by Concanavalin-A. Specifically, we provide evidence that the C/EBP alpha/miR-7 axis negatively controls CD4(+) T-cell activation and function through MAPK4, thereby orchestrating experimental AIH in mice. Conclusions This study expands on the important role of miR-7 in liver-related diseases and reveals the value of the C/EBP alpha/miR-7 axis in CD4(+) T-cell biological function for the pathogenesis of immune-mediated liver diseases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据