Multiple diversity facets of crucial microbial groups in biological soil crusts promote soil multifunctionality

Aim Microbial diversity is one of the most important factors for maintaining the performance of multiple functions in soils (multifunctionality). However, existing studies typically consider the taxonomic richness or Shannon diversity of the entire community. We know little about the connection network of taxonomic and phylogenetic facets of diversity and their collective impact on multifunctionality. In this study, the linkages of effects of diversity within functional groups were disentangled in drylands. Location The central Tibetan Plateau. Time period Present. Major taxa studied Phototrophs and diazotrophs (mainly cyanobacteria). Methods Given the carbon- and nitrogen-limited nature of drylands, we conducted a high-throughput sequencing of C/N-fixing functional groups from biocrusts and evaluated multiple facets of diversity (i.e., richness, evenness and phylogeny-related trait dissimilarity), together with seven crucial variables of soil functioning to calculate multifunctionality. The relationships between multifaceted diversity and abundance with individual functions and multifunctionality were validated by a set of solid statistical analyses. Results We found that the integrated biodiversity index was a stronger predictor of multifunctionality than richness. The divergent performance of different facets of diversity determined the idiosyncratic effect of each functional group on soil multifunctionality. Moreover, the evaluation of functional significance at the species level gave important clues on the trade-offs and redundancy in each functional group, explaining the distinct patterns of effects of diversity. Richness was the dominant factor for diazotrophs to maximize multifunctionality, whereas phylogenetic dissimilarity was the essential one for phototrophs. Main conclusions Our study demonstrated that multiple facets of diversity should be considered when assessing the effects of biodiversity. In contrast to the community level, within-functional group measures might adequately capture the features of diversity that are most correlated with soil multifunctionality. Our results provide a perspective to bridge the gap between taxonomic and trait-based approaches for elucidating the biodiversity-ecosystem function relationship.

Automated summary

This study in drylands disentangled the linkages of effects of diversity within functional groups and found that richness and phylogenetic dissimilarity were significant factors influencing soil multifunctionality. Evaluating functional significance at the species level provided important clues on the trade-offs and redundancy within each functional group, explaining the distinct patterns of effects of diversity.