期刊
GENETIC RESOURCES AND CROP EVOLUTION
卷 68, 期 5, 页码 1759-1767出版社
SPRINGER
DOI: 10.1007/s10722-021-01178-5
关键词
Chemical mutagenesis; Peanut; Virginia type; Export; High oleic; FAD2
资金
- Taishan Industry Leading Talents Special Fund, Yantai Science and Technology Plan Project [2018ZDCX]
- Corps Science and Technology Development Special Promotion Achievement Transformation Guidance Plan [2018BC012]
- China Agricultural Research System [CARS-13]
A high-oleic Virginia type peanut mutant was identified in this study, with mutations detected in the FAD2A/FAD2B genes. Sixteen promising lines have been bred and will undergo further yield tests after seed increase.
Less prone to oxidation than its conventional counterpart, high-oleic peanut (Arachis hypogaea L.) containing >= 72% oleate and < 8% linoleate is preferred by processors, seed sellers and consumers. Development of high-oleic peanut cultivars with suitable pod/seed size and shape may satisfy the need from food processors manufacturing whole kernel or halve products. In this study, a high-oleic Virginia type peanut mutant was identified through screening of a 15 mmol/L sodium azide mutagenized M3 population with near infrared spectroscopy. Sequencing of the mutated and wild type FAD2A/FAD2B genes detected 2 point mutations. The G448A mutation in FAD2A was the same as in previous reports, causing an amino acid change of D150N. G558A in FAD2B was a novel mutation, resulting in a stop codon and premature termination of protein synthesis. 16 promising lines with acceptable productivity and pod/seed characters have been tentatively bred, which will be evaluated further in yield tests with replications after seed increase.
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