Comparing of backfat microRNAomes of Landrace and Neijiang pig by high-throughput sequencing

Background MicroRNAs (miRNAs) could regulate the expression of target genes and play important roles in modulation of various metabolic processes. Nevertheless, little is known about the backfat microRNAome (miRNAome) of the Neijiang pig. Objectives The primary objective of this study was to analyse miRNAomes of Landrace and Neijiang pig backfat (LPB and NPB resp.). Furthermore, investigating differentially expressed miRNAs participating in lipid metabolism and mining potential biomarker for Neijiang pig breeding. Methods Here we used the Landrace pig with different metabolic characteristics as a control to analyse the Neijiang pig-specific backfat miRNAome. A comprehensive analysis of miRNAomes was performed by deep sequencing. Results Small RNA sequencing identified 326 unique miRNAs, 280 were co-expressed in both libraries. Only 11 and 35 miRNAs were specifically expressed in LPB and NPB respectively. Sixty seven differentially expressed miRNAs were identified by IDEG6. MiR-1-3p were identified that may participate in lipid metabolism. Furthermore, qPCR results revealed that lower expression of miR-1-3p in NPB could increase the expression of LXR alpha, which is an enzyme important for the synthesis and accumulation of lipid. The double luciferase report experiment suggested that LXR alpha was the direct target gene of miR-1-3p. In short, miR-1-3p could modulate the synthesis and accumulation of lipid by target LXR alpha. It may be a potential marker for pig breeding. Conclusion Our investigation has delineated the different miRNAs expression patterns of LPB and NPB, which may help understand the regulatory mechanisms of miRNAs in the lipid metabolism, and provide potential biomarkers for Neijiang pig breeding.

Automated summary

This study analyzed the miRNAomes of Landrace and Neijiang pig backfat, identified differentially expressed miRNAs related to lipid metabolism, and suggested a potential biomarker for Neijiang pig breeding.