期刊
GENES & DEVELOPMENT
卷 35, 期 9-10, 页码 698-712出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.348431.121
关键词
Spt6; Spn1; FACT; histone chaperones; transcription; Chromatin
资金
- National Institutes of Health (NIH) [F32GM119291, R01GM032967, R01GM120038, F31GM112370, R01HG007173]
- Ford Foundation Predoctoral Fellowship
- Research Computing Group at Harvard Medical School
Essential histone chaperones, Spt6, Spn1/Iws1, and FACT, interact with each other physically and functionally, with novel mutations in FACT identified in this study that do not restore the Spt6-Spn1 interaction. The suppressor mutations in FACT suggest alterations in FACT-nucleosome interactions, highlighting the critical role of histone chaperones in maintaining chromatin integrity during essential cellular processes.
Histone chaperones are critical for controlling chromatin integrity during transcription, DNA replication, and DNA repair. Three conserved and essential chaperones, Spt6, Spn1/Iws1, and FACT, associate with elongating RNA polymerase II and interact with each other physically and/or functionally; however, there is little understanding of their individual functions or their relationships with each other. In this study, we selected for suppressors of a temperature-sensitive spt6 mutation that disrupts the Spt6-Spn1 physical interaction and that also causes both transcription and chromatin defects. This selection identified novel mutations in FACT. Surprisingly, suppression by FACT did not restore the Spt6-Spn1 interaction, based on coimmunoprecipitation, ChIP, and mass spectrometry experiments. Furthermore, suppression by FACT bypassed the complete loss of Spn1. Interestingly, the FACT suppressor mutations cluster along the FACT-nucleosome interface, suggesting that they alter FACT-nucleosome interactions. In agreement with this observation, we showed that the spt6 mutation that disrupts the Spt6-Spn1 interaction caused an elevated level of FACT association with chromatin, while the FACT suppressors reduced the level of FACT-chromatin association, thereby restoring a normal Spt6-FACT balance on chromatin. Taken together, these studies reveal previously unknown regulation between histone chaperones that is critical for their essential in vivo functions.
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