期刊
GENERAL AND COMPARATIVE ENDOCRINOLOGY
卷 315, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygcen.2021.113796
关键词
Pro-opiomelanocortin; Adrenocorticotropic hormone; Ayu; Monocytes/macrophages; Phagocytosis; Anti-inflammation
资金
- Program for the National Natural Science Foundation of China [41776151, 31972821]
- Zhejiang Provincial Natural Science Foundation of China [LR18C040001, LZ18C190001]
- Fundamental Research Funds for the Provincial Universities of Zhejiang [SJLZ2020002]
This study discovered two isoforms of ACTH in the immune system of ayu, a type of fish. The two isoforms, PaACTH-A and PaACTH-B, have different immune functions. PaACTH-A shows anti-inflammatory effects and PaACTH-B enhances the bactericidal capacity of macrophages.
Adrenocorticotropic hormone (ACTH), a bioactive peptide of the family of melanocortins, is generated from proopiomelanocortin (POMC). So far, the research on the specific functions of ACTH in the immune system of teleosts is limited. We determined two complementary DNA (cDNA) sequences of POMC in ayu (Plecoglossus altivelis), termed PaPOMC-A and PaPOMC-B. PaPOMCs transcripts occurred in all examined tissues, and their expression in immune tissues changed following experimental infection with Vibrio anguillarum. PaACTH-B, but not PaACTH-A, suppressed the phagocytosis of monocytes/macrophages (MO/M Phi). Two isoforms of PaACTH increased the bactericidal capacity of MO/M Phi. PaACTH-A increased anti-inflammatory cytokine expression, while PaACTH-B decreased pro-inflammatory cytokine expression in MO/M Phi. Compared with PaACTH-B treatment, the PaACTH-A treatment improved survival rate and reduced the bacterial load in V. anguillarum-infected ayu through interleukin (IL)-10. Our results indicate that the two PaACTH isoforms exert different effects in the host defense against bacterial infection.
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