期刊
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
卷 46, 期 2, 页码 103-119出版社
SPRINGER
DOI: 10.1007/s00249-016-1155-9
关键词
Electroformation; Liposome; Peptide-induced fusion; Reconstitution; Vesicle
类别
资金
- Danish Council for Independent Research \ Natural Sciences (FNU) [1323-00297]
- Research Centre 'bioSYN-ergy' - UCPH Excellence Programme for Interdisciplinary Research
- Villum Foundation [VKR023115]
- Danish National Research Foundation through the PUMPKIN Centre of Excellence [DNRF85]
Studying membrane proteins at the molecular level represents a major challenge in biochemistry due to the complexity of the membrane in which they are embedded. As an important step towards a detailed understanding of their action and molecular functioning, current studies focus on membrane proteins reconstituted into artificial lipid environments. Such reconstituted systems allow for a more flexible choice of biochemical, biophysical, and microscopy techniques for characterizing the proteins. This review gives an overview of the methods currently available for reconstituting membrane proteins in a functional state into giant unilamellar vesicles, and discusses some key methods to verify successful reconstitution.
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