Antigenotoxic potential of the fermentation broth produced by Paenibacillus polymyxa RNC-D in vitro

Aim: Evaluate the chemopreventive potential of the extract from P. polymyxa RNC-D. Methods: Concentrations of P. polymyxa RNC-D extract were tested in HepG2/C3A cells to assess their genotoxic (comet assay), mutagenic (micronucleus test) and antigenotoxic potential (comet assay) in vitro. Results: 400 and 40 mu g/ml concentrations induced DNA lesions, whereas the 4 mu g/ml induced a desmutagenic effect. Complementary tests indicated that the extract minimized the formation of reactive oxygen species induced by methyl methanesulfonate and normalized the loss of membrane potential. The quantification of cytokines indicated that TNF-alpha was immunostimulated by the extract. However, when administered in conjunction with the methyl methanesulfonate, the extract blocked the TNF-alpha release. Conclusion: The fermentation broth from P. polymyxa RNC-D showed an antigenotoxic effect, and thus the potential to be used as chemopreventive compound.

Automated summary

The extract from P. polymyxa RNC-D showed antigenotoxic potential by inhibiting DNA lesions and minimizing the formation of reactive oxygen species. It also regulated cytokine release in cell cultures.