Analysis of melanin biosynthesis in the plant pathogenic fungus Colletotrichum gloeosporioides

Melanin is recognized as a dark pigment that can protect fungi from the harm of environmental stresses. To investigate what roles of melanin played in the pathogenicity and development of Colletotrichum gloeosporioides, a causal agent of poplar anthracnose, genes encoding a transcription factor CgCmr1 and a polyketide synthase CgPks1 were isolated as the ortholog of Magnaporthe oryzae Pig1 and Pks1 respectively. Deletion of CgCmr1 or CgPks1 resulted in melanin-deficient fungal colony. The DCgPks1 mutant showed no melanin accumulation in appressoria, and lack of CgCmr1 also resulted in the delayed and decreased melanization of appressoria. In addition, the turgor pressure of the appressorium was lower in DCgPks1 and DCgCmr1 than in the wild-type (WT). However, DHN melanin was not a vital factor for virulence in C. gloeosporioides. Moreover, deletion of CgCmr1 and CgPks1 resulted in the hypersensitivity to hydrogen peroxide (H2O2) oxidative stress but not to other abiotic stresses. Collectively, these results suggest that CgCmr1 and CgPks1 play an important role in DHN melanin biosynthesis, and melanin was not an essential factor in penetration and pathogenicity in C. gloeosporioides. The data presented in this study will facilitate future evaluations of the melanin biosynthetic pathway and development in filamentous fungi. (c) 2021 British Mycological Society. Published by Elsevier Ltd. All rights reserved.

Automated summary

In Colletotrichum gloeosporioides, CgCmr1 and CgPks1 play important roles in DHN melanin biosynthesis, but melanin is not essential for penetration and pathogenicity. Deletion of CgCmr1 and CgPks1 results in hypersensitivity to hydrogen peroxide oxidative stress, but not to other abiotic stresses.