期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 166, 期 -, 页码 104-115出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2021.02.014
关键词
Noptosis; Glioblastoma; Tanshindiol B; NQO1; JNK1; 2
资金
- Science and Technology Development Fund, Macau SAR [175/2017/A3]
- University of Macau [MYRG202000053ICMS]
In this study, tanshindiol B (TSB) inhibited the growth of glioblastoma by inducing a type of necrosis known as noptosis, which showed anti-tumor potential both in vitro and in vivo. TSB activated the NQO1 enzyme activity, leading to oxidative stress-induced necrotic cell death. These effects were mediated by c-Jun N-terminal kinase and completely abolished by a specific NQO1 inhibitor, dicoumarol.
Glioblastoma (GBM) is one of the most prevalent malignant primary tumors in the human brain. Temozolomide (TMZ), the chemotherapeutic drug for GBM treatment, induces apoptosis. Unfortunately, apoptosis-resistance to TMZ results in treatment failure. GBM shows enhanced expression of NAD(P)H: quinone oxidoreductase 1 (NQO1). Recently, noptosis, a type of NQO1-dependent necrosis, was proposed. Here, we identified that tanshindiol B (TSB) inhibits GBM growth by induction of noptosis. TSB triggered significant cell death, which did not fit the criteria of apoptosis but oxidative stress-induced necrosis. Molecular docking, cellular thermal shift assay, and NQO1 activity assay revealed that TSB bind to and promptly activated NQO1 enzyme activity. As the substrate of NQO1, TSB induced oxidative stress, which resulted in dramatic DNA damage, poly (ADP-ribose) polymerase 1 (PARP1) hyperactivation, and NAD+ depletion, leading to necrotic cell death. These effects of TSB were completely abolished by specific NQO1 inhibitor dicoumarol (DIC). Furthermore, the c-Jun N-terminal kinase 1/2 (JNK1/2) plays an essential role in mediating TSB-induced cell death. Besides, TSB significantly suppressed tumor growth in a zebrafish xenograft model mediated by NQO1. In conclusion, these results showed that TSB was an NQO1 substrate and triggered noptosis of GBM. TSB exhibited anti-tumor potentials in GBM both in vitro and in vivo. This study provides a novel strategy for fighting GBM through the induction of noptosis.
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