4.4 Article

Development and validation of multiplex PCR assay for differentiating tunas and billfishes

期刊

FOOD SCIENCE AND BIOTECHNOLOGY
卷 30, 期 4, 页码 497-503

出版社

KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
DOI: 10.1007/s10068-021-00893-0

关键词

Tunas; Billfishes; PCR assay; Validation; Monitoring

资金

  1. Ministry of Food and Drug Safety in 2019 [17162 MFDS 064]

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Species-specific primers were developed to differentiate between tunas and billfishes, and a multiplex PCR assay was used to determine the authenticity of these species. The results showed that the developed primers were effective in distinguishing between the two species, making it a cost-effective technique for species identification.
Commercially available tunas and billfishes are generally processed as steaks, making it difficult to visually distinguish between the two. We developed and validated species-specific primers to prevent the adulteration of tunas by billfishes. Tunas and billfishes primers were designed on the cytochrome oxidase subunit I. Multiplex PCR bands obtained were 579 bp, 291 bp and 114 bp for tunas, billfishes and internal control. Sensitivity was determined to be 5 ng for tunas and billfishes. A total of 50 samples were monitored: 49 for tunas and 1 for billfish. As a result of the monitoring, the fake tunas did not show due to the agreement between product name and the raw material of the wrapping paper. Our results indicate that the species-specific primers developed in this study are suitable for differentiating tunas and billfishes. The newly developed multiplex PCR assay is a time and cost effective technique for determining the authenticity of tunas and billfishes.

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