期刊
FOOD CHEMISTRY
卷 345, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.128855
关键词
Soy protein isolate; Angiotensin-I converting enzyme inhibitor; Activated carbon; Molecular docking; Simulated gastrointestinal digestion
资金
- Special Support Project of Guangdong Province for Science and Technology Innovative Young Talents [2016TQ03N728]
- Fundamental Research Funds for the Central Universities
- Natural Science Foundation of Guangdong Province of China [2020A1515010659]
This study investigates natural angiotensin-I converting enzyme (ACE) inhibitors by preparing soybean protein isolated (SPI) hydrolysate (SPIH) through Alcalase hydrolysis, and obtaining ACE inhibitory peptides through membrane separation, ethanol precipitation, and adsorption chromatography enrichment. Activated carbon is more suitable for peptide enrichment than eight macroporous resins, and peptides like IY, YVVF may be the main contributors to SPIH's ACE inhibition with high ACE inhibitory activity.
This study attempts to investigate natural angiotensin-I converting enzyme (ACE) inhibitors. Soybean protein isolated (SPI) hydrolysate (SPIH) was prepared by Alcalase from inexpensive SPI, and their ACE inhibitory peptides were obtained via membrane separation, ethanol precipitation and adsorption chromatography enrichment. Activated carbon was more suitable for peptide enrichment than eight macroporous resins. The peptide fraction yielded under optimal conditions (protein-active carbon mass ratio 2:1; adsorption pH 3.0 and time 2 h; desorption time 2 h) exhibited a 10.4 times higher ACE-inhibitory activity than SPIH. Novel peptides IY, YVVF, LVF, WMY, LVLL and FF (hydrophobicity values 10.51-12.87; activity scores 0.2373-0.999) might be the main contributors to SPIH's ACE inhibition. IY had the lowest IC50 (0.53 +/- 0.02 mu M). YVVF had the greatest affinity (-9.8 kcal/mol) for 2OC2 (ACE's C-domain receptor) via H-bonds. IY and WMY could be potent ACE inhibitors, and their ACE-inhibitory activities unaltered and increased after in vitro gastrointestinal digestion.
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