期刊
FEMS MICROBIOLOGY ECOLOGY
卷 97, 期 6, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/femsec/fiab060
关键词
Qinghai Tibet Plateau; alpine grasslands; seed microbiome; endophytes; plant growth promotion; illumina sequencing
类别
资金
- Lanzhou University [lzujbky2017-ot21]
- Project of the Second Tibetan Plateau Scientific Expedition [2019QZKK0302]
- National Natural Science Foundation of China [31672472]
- programfor Changjiang Scholars and Innovative Research Team in University [IRT 17R50]
Seed borne microorganisms in native alpine grassland plants were characterized, revealing diverse bacterial populations with various functions. Plant species identity was found to be a key determinant shaping the seed bacterial community.
Seed borne microorganisms play an important role in plant biology. Concerns have recently been raised about loss of seed microbial diversity by seed treatments, crop domestication and plant breeding. Information on the seed microbiomes of native plants growing in natural ecosystems is beneficial as they provide the best settings to detect indigenous plant microbe interactions. Here, we characterized the seed bacterial community of 8 native alpine grassland plants. First, seed bacterial diversity was examined using Illumina DNA sequencing, then 28 cultivable bacteria were isolated and potential functions were explored. Across 8 plant species, 343 different bacterial genera were identified as seed endophytes, 31 of those were found in all plant species, indicating a high level of conservation. Proteobacteria, Actinobacteria, Firmicutes, Bacteroidetes and Chloroflexi were the top five dominant phyla. Plant species identity was a key determinant shaping the seed endophytic bacteriome. ACC deaminase activity, siderophores production and secretion of lytic enzymes were common functions shown by isolated bacteria. Our results demonstrate that highly diverse and beneficial bacterial populations are hosted by seeds of alpine grassland species to ensure the establishment of best bacterial symbionts for the next generation. This information is useful for crop improvement by reinstating beneficial seed microbial diversities for high-quality forage and crop seeds.
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