Global transcriptomic analysis of a murine osteocytic cell line subjected to spaceflight

Bone loss is a major health concern for astronauts during long-term spaceflight and for patients during prolonged bed rest or paralysis. Growing evidence suggests that osteocytes, the most abundant cells in the mineralized bone matrix, play a key role in sensing mechanical forces applied to the skeleton and integrating the orchestrated response into subcellular biochemical signals to modulate bone homeostasis. However, the precise molecular mechanisms underlying both mechanosensation and mechanotransduction in late-osteoblast-to-osteocyte cells under microgravity (mu G) have yet to be elucidated. To unravel the mechanisms by which late osteoblasts and osteocytes sense and respond to mechanical unloading, we exposed the osteocytic cell line, Ocy454, to 2, 4, or 6 days of mu G on the SpaceX Dragon-6 resupply mission to the International Space Station. Our results showed that mu G impairs the differentiation of osteocytes, consistent with prior osteoblast spaceflight experiments, which resulted in the downregulation of key osteocytic genes. Importantly, we demonstrate the modulation of critical glycolysis pathways in osteocytes subjected to microgravity and discovered a set of mechanical sensitive genes that are consistently regulated in multiple cell types exposed to microgravity suggesting a common, yet to be fully elucidated, genome-wide response to microgravity. Ground-based simulated microgravity experiments utilizing the NASA rotating-wall-vessel were unable to adequately replicate the changes in microgravity exposure highlighting the importance of spaceflight missions to understand the unique environmental stress that microgravity presents to diverse cell types. In summary, our findings demonstrate that osteocytes respond to mu G with an increase in glucose metabolism and oxygen consumption.

Automated summary

Bone loss is a significant health concern for astronauts and patients in extended periods of bed rest or paralysis. Osteocytes play a key role in sensing and responding to mechanical forces applied to the skeleton, and under microgravity conditions, there is impairment in osteocyte differentiation and downregulation of key osteocytic genes. Additionally, a genome-wide response to microgravity including changes in glycolysis pathways and mechanical sensitive genes has been observed.