期刊
FASEB JOURNAL
卷 35, 期 5, 页码 -出版社
WILEY
DOI: 10.1096/fj.202002570RR
关键词
AhR; chromatin remodeling; CYP1A4; DNA methylation; T-2 toxin
资金
- Natural Science Foundation of Guangdong Province [2016A030313166, 2018A030313001]
- National Natural Science Foundation of China [U1901207]
- National Natural Science Foundation of Guangdong Province [U1901207]
- Science and Technology Program of Guangzhou [201804020067]
The study found that T-2 toxin and its metabolites induce the expression of cytochrome P450 1A4 gene, involving the regulation of aryl hydrocarbon receptor and DNA methylation. T-2 toxin activates AhR, leading to transcriptional activation of the cytochrome P450 1A4 gene.
Mycotoxins are toxic secondary metabolites produced by food-contaminating fungi, which lead to global epigenetic changes and cause toxicity to both farm animals and humans. However, whether mycotoxins induce gene-specific epigenetic alterations associated with inducible downstream gene expression is unclear as are the underlying regulatory mechanisms. Here, we found that T-2 toxin and its deacetylated metabolites but not deoxynivalenol (DON) or other representative mycotoxins highly induced the expression of cytochrome P450 1A4 (CYP1A4) in both Leghorn male hepatoma (LMH) cells and chicken primary hepatocytes, and this effect was related to the regulation of both aryl hydrocarbon receptor (AhR) and DNA methylation. We used methylation-sensitive restriction enzyme digestion-qPCR (MSRE-qPCR) and chromatin immunoprecipitation (ChIP) assays and found that the binding of DNA methyltransferase 1 (DNMT1) and histone deacetylase 2 (HDAC2) to highly methylated CpG island 3-2 at the enhancer of CYP1A4 was accompanied by the recruitment of the repressive histone modification marker H3K27me3, inducing a silent state. In turn, T-2 toxin stimulation enriched the binding of AhR to demethylated CpG island 3-2, which facilitated p300 and H3K9ac recruitment and ultimately generated an activated chromatin structure at the enhancer by increasing the active histone modification markers, including H3K4me3, H3K27ac, and H3K14ac. Interestingly, T-2 toxin-induced AhR activation also facilitated RNA polymerase II binding to CpG island 2, which may form a transcriptionally active chromatin structure at the promoter and ultimately transactivate CYP1A4. Our findings provide novel insights into the epigenetic regulation of T-2 toxin-induced gene expression.
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