期刊
BRAIN RESEARCH
卷 1615, 期 -, 页码 89-97出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.brainres.2015.04.033
关键词
Thioredoxin; Middle cerebral artery occlusion (MCAO); Apoptosis; Oxidative stress
资金
- National Natural Science Foundation of China [81222015]
- New Century Talent Supporting Project by Chinese Education Ministry [NECT-12-1004]
- Leading Talents of Middle-age and Young in S&T Innovation - Science and Technology Ministry of China [2013RA2181]
Recent studies demonstrate that Thioredixin (Trx) possesses a neuronal protective effect and closely relates to oxidative stress and apoptosis of cerebral ischemia injury. The present study was conducted to validate the neuroprotective effect of recombinant human Trx-1 (rhTrx-1) and its potential mechanisms against ischemia injury at middle cerebral artery occlusion (MCAO) in mice. rhTrx-1 was administrated intraperitoneally at a dose of 5, 10 and 20 mg/kg 30 mm before MCAO in mice, and its neuronal protective effect was evaluated by neurological deficit score, brain dry-wet weight, 2,3,5-triphenyltetrazolium chloride (TTC) staining. The protein carbonyl content and HO-1 were detected to investigate its potential anti-oxidative and anti-inflammatory property, and the antiapoptotic ability of rhTrx-1 was assessed by casepase-3 and TUNEL staining. The results demonstrated that rhTrx-1 significantly improved neurological functions and reduced cerebral infarction and apoptotic cell death at 24 h after MCAO. Moreover, rhTrx-1 resulted in a significant decrease in carbonyl contents and HO-1 against oxidative stress, which turned to be fast reduction during the first 24 h and tended to be stable from 24 h to 72 h after MCAO. The study shows that rhTrx-1 exerts an neuroprotective effect in cerebral ischemia injury. The anti-oxidative, anti-apoptotic and anti-inflammatory properties of rhTrx-1 are more likely to succeed as a therapeutic approach to diminish oxidative stress-induced neuronal apoptotic cell death in acute ischemic stroke. (C) 2015 Elsevier B.V. All rights reserved.
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