期刊
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
卷 50, 期 3-4, 页码 661-670出版社
SPRINGER
DOI: 10.1007/s00249-021-01518-4
关键词
Isothermal titration calorimetry; Confidence intervals; Standard error; Log-normal distribution; Dissociation constant; Binding constant
类别
资金
- Research Council of Lithuania [S-LLT-20-2]
- Fundacao para a Ciencia e Tecnologia (FCT), Portugal [UIDB/00081/2020, UIDB/00313/2020]
- COST action ARBRE-MOBIEU by COST (European Cooperation in Science and Technology) [CA15126]
The passage discusses the importance of equilibrium binding constants and uncertainties in scientific decision-making. It highlights limitations of using symmetric normal distributions to represent uncertainties in measured K-b values and suggests reporting 95% confidence intervals for a more accurate representation of uncertainties in K-b, K-d, and Delta G(b) values. The passage also illustrates the effect of asymmetric K-b distributions and the changing degree of asymmetry when transforming distributions of K-b, K-d, and Delta G(b) values.
Equilibrium binding constants (K-b) between chemical compounds and target proteins or between interacting proteins provide a quantitative understanding of biological interaction mechanisms. Reported uncertainties of measured experimental parameters are critical for decision-making in many scientific areas, e.g., in lead compound discovery processes and in comparing computational predictions with experimental results. Uncertainties in measured K-b values are commonly represented by a symmetric normal distribution, often quoted in terms of the experimental value plus-minus the standard deviation. However, in general, the distributions of measured K-b (and equivalent K-d) values and the corresponding free energy change Delta G(b) are all asymmetric to varying degree. Here, using a simulation approach, we illustrate the effect of asymmetric K-b distributions within the realm of isothermal titration calorimetry (ITC) experiments. Further we illustrate the known, but perhaps not widely appreciated, fact that when distributions of any of K-b, K-d and Delta G(b) are transformed into each other, their degree of asymmetry is changed. Consequently, we recommend that a more accurate way of expressing the uncertainties of K-b, K-d, and Delta G(b) values is to consistently report 95% confidence intervals, in line with other authors' suggestions. The ways to obtain such error ranges are discussed in detail and exemplified for a binding reaction obtained by ITC.
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