Influence of foliar glutathione and putrescine on metabolism and mineral status of genetically diverse rapeseed cultivars under hexavalent chromium stress

We studied the physio-biochemical involvement of exogenous signaling compounds, glutathione and putrescine (alone and in combination), on three contrasting genotypes (cvs. Shiralee, Rainbow, and Dunkled) of canola (Brassica napus L.) of plants exposed to chromium stress. Seeds were germinated in Cr-contaminated soil (0 and 50 mu g/g Cr6+), and both signaling compounds were applied as a foliar spray to 20-day-old plants. Changes in root, stem, and leaf nitro-oxidative metabolism, endogenous GSH level, secondary metabolites, and mineral nutrients were investigated from 60-day-old plants. Exposure to Cr6+ increased stem GSH and NO concentrations in all cultivars. Maximum root Cr6+ bioaccumulation was recorded in cv. Rainbow and the least in cv. Shiralee. Also, Cr6+ stress decreased number and weight of seeds and pod length. Disturbances in root and shoot mineral profile were evident; however, its magnitude varied in all cultivars. The exogenous GSH improved root and shoot P, Fe, S, and Zn concentrations; however, the effect was cultivar specific. Leaf endogenous GSH was increased by exogenous GSH while NO levels remained unaffected. The GSH application also promoted shoot Cr6+ bioaccumulation while PUT application caused a recovery in seed number and seed weight. Both PUT and GSH differentially affected tissue-specific secondary metabolite profile. Overall, the exogenous GSH was much more effective in alleviating the Cr+6 toxicity in canola.

Automated summary

The study investigated the physio-biochemical involvement of exogenous signaling compounds on different genotypes of canola exposed to chromium stress, with exogenous glutathione proving to be more effective in alleviating Cr+6 toxicity and showing cultivar-specific effects.