期刊
ELECTROCHIMICA ACTA
卷 386, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.electacta.2021.138480
关键词
Nitrate biosensor; Voltammetry; Chitosan; Amperometry
资金
- Australian Research Council [DP190103158]
The electrochemical nitrate biosensor utilizes nitrate reductase from Neurospora crassa, entrapped within a polymeric chitosan matrix on a glassy carbon electrode, with the electron mediator anthraquinone sulfonate. The sensor shows high sensitivity, selectivity, and long-term stability, and is successfully applied for nitrate detection in lake and river water samples.
We report an electrochemical nitrate biosensor incorporating the enzyme nitrate reductase (NR) from the fungus Neurospora crassa entrapped within a polymeric chitosan matrix on a glassy carbon (GC) electrode. The artificial electron mediator anthraquinone sulfonate (AQ) is utilized as an electron transfer partner (co-substrate) for NR. The NR enzyme was effectively immobilized within the chitosan matrix without the need for a dialysis membrane, and exhibited high sensitivity and selectivity for nitrate using either rotating disk cyclic voltammetry or constant potential amperometry methods. A particular feature was the longevity of the biosensor which retained more than 70% activity over a period of 3 months. The practical application of the biosensor was demonstrated by the determination of nitrate concentrations in lake and river water samples without any pre-treatment, and the results were validated with a reliable spectroscopic assay. (c) 2021 Elsevier Ltd. All rights reserved.
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