期刊
DIGESTIVE DISEASES AND SCIENCES
卷 67, 期 6, 页码 2420-2432出版社
SPRINGER
DOI: 10.1007/s10620-021-07000-7
关键词
Gut microbiota; Critical care; 16S rRNA; Dysbiosis; Antibiotics; Sequential organ failure assessment score
资金
- Japan Society for the Promotion of Science (JSPS) KAKENHI [17K17049, 19H03760, 19H03761]
- Grants-in-Aid for Scientific Research [17K17049, 19H03760, 19H03761] Funding Source: KAKEN
This study used 16S rRNA gene deep sequencing to evaluate changes in gut microbiota of ICU patients, finding a convergence and stabilization of Bacteroidetes and Firmicutes proportions within the first week of admission. It also suggested that broad-spectrum antibiotics and disease severity may be associated with gut dysbiosis in ICU patients, and that dysbiosis in the gut may be linked to mortality in ICU patients.
Background The gut microbiota are reported to be altered in critical illness. The pattern and impact of dysbiosis on prognosis has not been thoroughly investigated in the ICU setting. Aims We aimed to evaluate changes in the gut microbiota of ICU patients via 16S rRNA gene deep sequencing, assess the association of the changes with antibiotics use or disease severity, and explore the association of gut microbiota changes with ICU patient prognosis. Methods Seventy-one mechanically ventilated patients were included. Fecal samples were collected serially on days 1-2, 3-4, 5-7, 8-14, and thereafter when suitable. Microorganisms of the fecal samples were profiled by 16S rRNA gene deep sequencing. Results Proportions of the five major phyla in the feces were diverse in each patient at admission. Those of Bacteroidetes and Firmicutes especially converged and stabilized within the first week from admission with a reduction in alpha-diversity (p < 0.001). Significant differences occurred in the proportional change of Actinobacteria between the carbapenem and non-carbapenem groups (p = 0.030) and that of Actinobacteria according to initial SOFA score and changes in the SOFA score (p < 0.001). An imbalance in the ratio of Bacteroidetes to Firmicutes within seven days from admission was associated with higher mortality when the ratio was > 8 or < 1/8 (odds ratio: 5.54, 95% CI: 1.39-22.18, p = 0.015). Conclusions Broad-spectrum antibiotics and disease severity may be associated with gut dysbiosis in the ICU. A progression of dysbiosis occurring in the gut of ICU patients might be associated with mortality.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据