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Usefulness of the GenMark ePlex RPP assay for the detection of respiratory viruses compared to the FTD21 multiplex RT-PCR

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ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2021.115424

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ePlex RPP; FTD21 multiplex RT-PCR; respiratory viruses; syndromic testing; rapid diagnostics

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The study compared the performance of ePlex respiratory pathogen panel (RPP) and FTD21 multiplex RT-PCR assay, showing differences in sensitivity and specificity. The ePlex RPP is valuable for rapid detection of respiratory viruses, except for the coronavirus family and samples with low pathogen load.
Cartridge-based multiplex panels covering numerous pathogens offer an advantage of minimal hands-on -time and short time to result to commercial RT-PCR assays. In this study, we evaluated the performance of the ePlex respiratory pathogen panel (RPP) compared to the Fast Track Diagnostics Respiratory pathogens 21 multiplex RT-PCR assay (FTD21) using 400 clinical respiratory samples. Discrepant results were further analysed by a reference nucleic acid amplification testing (NAT) and a composite reference approach was used for final interpretation. Discordant results were observed in 56 targets corresponding to 54 samples. Sensitivities and specificities were 85.5% and 99.9% for the ePlex RPP and 95.8% and 99.7% for the FTD21 sys-tem, respectively. Altogether, the ePlex RPP is a valuable tool for the rapid detection of a number of different respiratory viruses with the exception of the coronavirus family (low sensitivity ranging from 50-80%) and samples with a low pathogen load (Ct values >33). (c) 2021 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)

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