The odd-skipped related gene drumstick is required for leg development in the beetle Tribolium castaneum

Background The evolutionarily conserved odd-skipped related genes odd-skipped (odd), drumstick (drm), sister of odd and bowel (sob), and brother-of-odd-with-entrails-limited (bwl) act downstream of the Notch pathway in various insect tissues including the appendages and the gut. While the function of some of these genes have been analyzed in the adult Tribolium beetle, the expression during and their requirement for embryonic development is not known. Results We describe here the embryonic expression patterns of drm, sob, and bwl and analyze the RNAi knockdown phenotypes with emphasize on the appendages and the hindgut. We show that in Tribolium, drm acts independently of other odd-family members in the formation of legs, hindgut, and the dorsal epidermis. Moreover, we establish drm and sob as further markers for segment borders in the appendages that include the gnathobasic mandibles. Conclusions We conclude that the regulatory interrelationship among the odd genes differs between Tribolium and Drosophila, where odd and drm seem to act redundantly. In Tribolium, the genes drm and sob uncover the relict of a precoxal joint incorporated in the lateral body wall.

Automated summary

The study revealed that in Tribolium, drm acts independently of other odd-family members in the formation of appendages and hindgut, while drm and sob are identified as further markers for segment borders in the appendages. Additionally, the regulatory interrelationship among the odd genes is found to differ between Tribolium and Drosophila.