The use of transgenics in the laboratory axolotl

The ability to generate transgenic animals sparked a wave of research committed to implementing such technology in a wide variety of model organisms. Building a solid base of ubiquitous and tissue-specific reporter lines has set the stage for later interrogations of individual cells or genetic elements. Compared to other widely used model organisms such as mice, zebrafish and fruit flies, there are only a few transgenic lines available in the laboratory axolotl (Ambystoma mexicanum), although their number is steadily expanding. In this review, we discuss a brief history of the transgenic methodologies in axolotl and their advantages and disadvantages. Next, we discuss available transgenic lines and insights we have been able to glean from them. Finally, we list challenges when developing transgenic axolotl, and where further work is needed in order to improve their standing as both a developmental and regenerative model.

Automated summary

The ability to generate transgenic animals has led to a surge of research in various model organisms, yet the availability of transgenic lines in laboratory axolotls remains limited compared to other commonly used model organisms. This review discusses the history, advantages, and disadvantages of transgenic methodologies in axolotls, as well as insights gained from existing transgenic lines. Challenges in developing transgenic axolotls and the need for further research to enhance their role as developmental and regenerative models are also addressed.