期刊
DEVELOPMENTAL CELL
卷 56, 期 7, 页码 1043-+出版社
CELL PRESS
DOI: 10.1016/j.devcel.2021.03.014
关键词
-
资金
- NIH Instrumentation grants [S10OD018220, 1S10OD021763]
- NIH-NIGMS Ruth L. Kirschstein-NRSA postdoctoral fellowship [F32GM129918]
- EMBO postdoctoral fellowship [ALTF 707-2012]
- Deutsche Forschungsgemeinschaft
The study investigates the development and regulation of stomatal lineage in Arabidopsis leaf epidermis, revealing detailed cell differentiation processes using single-cell transcriptomics and genetics. It identifies the extended role of transcriptional regulator in reinforcing cell fate commitment through integration of genome-scale datasets and precise functional manipulations.
Dynamic cell identities underlie flexible developmental programs. The stomatal lineage in the Arabidopsis leaf epidermis features asynchronous and indeterminate divisions that can be modulated by environmental cues. The products of the lineage, stomatal guard cells and pavement cells, regulate plant-atmosphere exchanges, and the epidermis as a whole influences overall leaf growth. How flexibility is encoded in development of the stomatal lineage and how cell fates are coordinated in the leaf are open questions. Here, by leveraging single-cell transcriptomics and molecular genetics, we uncovered models of cell differentiation within Arabidopsis leaf tissue. Profiles across leaf tissues identified points of regulatory congruence. In the stomatal lineage, single-cell resolution resolved underlying cell heterogeneity within early stages and provided a fine-grained profile of guard cell differentiation. Through integration of genome-scale datasets and spatiotemporally precise functional manipulations, we also identified an extended role for the transcriptional regulator SPEECHLESS in reinforcing cell fate commitment.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据