期刊
DENTAL MATERIALS JOURNAL
卷 40, 期 2, 页码 312-321出版社
JAPANESE SOC DENTAL MATERIALS DEVICES
DOI: 10.4012/dmj.2020-005
关键词
Electrospun; Polycaprolactone; Hydroxyapatite; Dentin sialophosphoprotein; Inflamed dental pulp stem cells
资金
- University of Sharjah [1901100237]
This study developed electrospun nanofibrous membranes blending PCL with HA, with PCL10%HA membrane showing the most favorable effects on cell attachment, upregulation of gene expression, and Ca/P ratio compared to PCL and PCL15%HA membranes in inducing differentiation of IDPSCs into odontoblasts.
The objective of this study was to develop electrospun polycaprolactone (PCL) membranes blended with hydroxyapatite (HA) and evaluate its potential in differentiating inflamed dental pulp stem/progenitor cells (IDPSCs) into odontoblasts. Electrospun nanofibrous membrane consisting of PCL blended with 10 wt% and 15 wt% of HA were fabricated and the characterization was done by Scanning electron microscopy (SEM), Fourier- transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and contact angle analysis. Cytocompatibility, cell adhesion and odontogenic differentiation ability of the membranes were assessed by MTT, Live/Dead, SEM/DAPI and qPCR studies. The mineral deposition ability of the membranes with IDPSCs was estimated by SEM-EDS. The SEM analysis revealed a nanofibrous texture with an average fiber diameter of 140 nm for PCL, 220 nm for PCL10%HA and 250 nm for PCL15%HA. Among the membranes tested, PCL10%HA favored positive cell attachments, upregulated expression of DSPP and ALP gene and higher Ca/P ratio compared to PCL and PCL15%HA.
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