期刊
CURRENT OPINION IN PLANT BIOLOGY
卷 60, 期 -, 页码 -出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.pbi.2020.101980
关键词
-
资金
- NSF Plant Genome Research Program [IOS-1758745, IOS-2029889]
- USDA-NIFA Biotechnology Risk Assessment Grant Program [2018-33522-28789, 2020-33522-32274]
- USDA-NIFA Emergency Citrus Disease Research and Extension Program [2020-70029-33161]
- FFAR New Innovator in Food and Agriculture Research Award [593603]
- Syngenta
The precise genome editing mediated by the CRISPR/Cas system has revolutionized genome engineering, with the use of dCas variants expanding its applications to genetic and translational research without inducing DNA double-strand breaks. Fusion of effector domains to dCas proteins empowers the CRISPR/dCas system as a multifunctional platform, enabling gene expression regulation, epigenetic regulation, and sequence-specific imaging. This review highlights recent advances in transcriptional activation and repression, as well as epigenetic modifications, and discusses the future directions and broader applications of the CRISPR/dCas systems in plants.
The CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR Associated) system-mediated precise genome editing has revolutionized genome engineering due to ease of use and versatility of multiplexing. Catalytically inactivated Cas variants (dCas) further expand the usefulness of the CRISPR/Cas system for genetics studies and translational research without inducing DNA double-strand breaks. Fusion of diverse effector domains to dCas proteins empowers the CRISPR/dCas system as a multifunctional platform for gene expression regulation, epigenetic regulation and sequence-specific imaging. In this short review, we summarize the recent advances of CRISPR/dCas-mediated transcriptional activation and repression, and epigenetic modifications. We also highlight the future directions and broader applications of the CRISPR/dCas systems in plants.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据