Difructose anhydride III: a 50-year perspective on its production and physiological functions

Production and applications of difructose anhydride III (DFA-III) have attracted considerable attention because of its versatile physiological functions. Recently, large-scale production of DFA-III has been continuously explored, which opens a horizon for applications in the food and pharmaceutical industries. This review updates recent advances involving DFA-III, including: biosynthetic strategies, purification, and large-scale production of DFA-III; physiological functions of DFA-III and related mechanisms; DFA-III safety evaluations; present applications in food systems, existing problems, and further research prospects. Currently, enzymatic synthesis of DFA-III has been conducted both industrially and in academic research. Two biosynthetic strategies for DFA-III production are summarized: single- and double enzyme-mediated. DFA-III purification is achieved via yeast fermentation. Enzyme membrane bioreactors have been applied to meet the large-scale production demands for DFA-III. In addition, the primary physiological functions of DFA-III and their underlying mechanisms have been proposed. However, current applications of DFA-III are limited. Further research regarding DFA-III should focus on commercial production and purification, comprehensive study of physiological properties, extensive investigation of large-scale human experiments, and expansion of industrial applications. It is worthy to dig deep into potential application and commercial value of DFA-III.

Automated summary

The production and applications of difructose anhydride III (DFA-III) have attracted considerable attention due to its versatile physiological functions. Recent advances include biosynthetic strategies, purification methods, physiological functions, safety evaluations, current applications, and future research prospects. Enzymatic synthesis of DFA-III has been conducted in both industrial and academic research, with purification achieved through yeast fermentation. Further research should focus on commercial production, comprehensive study of physiological properties, large-scale human experiments, and expansion of industrial applications to explore the potential benefits of DFA-III.