期刊
BRAIN INJURY
卷 29, 期 11, 页码 1376-1382出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/02699052.2015.1042053
关键词
A beta 1-40; astrocyte; GSH-Px; LDH; MDA; okadaic acid; neuropathology; SSF
资金
- Hebei Provincial Education Department [ZD20131022]
- Hundred Outstanding Innovated Talents, Hebei Province
- Key Subject Construction Project of Hebei Provincial College of China
Primary objective: To study the effect of flavonoids isolated from aerial parts of Scutellaria baicalensis Georgi (SSF) on cerebral damage induced by okadaic acid (OA) in rats. Methods and procedures: OA was microinjected into the right lateral ventricle of male rats at a dose of 200 ng kg(-1) twice with a 3-day interval between injections to establish a model of Alzheimer's-disease-like cerebral damage. Neuronal morphology was observed with thionin staining and the expressions of glial fibrillary acidic protein (GFAP) and b-amyloid peptide 1-40 (Ab1-40) were monitored via immunohistochemistry. The level of malondialdehyde (MDA) and the activities of glutathione peroxidase (GSH-Px) and lactate dehydrogenase (LDH) were measured using spectrophotometry. Main outcomes and results: The results showed that OA-treated rats exhibited marked neuronal damage accompanied by increased levels of Ab1-40 peptide and MDA accumulation, decreased GFAP protein expression and reduced GSH-Px and LDH activity in the brain. SSF at three doses (25, 50 and 100 mg kg(-1)) dramatically reversed the OA-induced changes in the brains of rats. Conclusion: SSF-mediated amelioration of OA-induced neuronal damage in rats provides a rationale for assessing SSF as a means of to reducing tau hyperphosphorylation and Ab expression in the treatment of Alzheimer's disease.
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