期刊
CHINESE CHEMICAL LETTERS
卷 32, 期 5, 页码 1827-1830出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2020.11.031
关键词
Aptazyme; Exosomes; Fluorescence probe; Signal amplification; Clinical serum samples
资金
- National Natural Science Foundation of China [21605038, 21974125]
- China Postdoctoral Science Foundation [2019T120623]
A molecular recognition triggered aptazyme cascade strategy was developed for ultrasensitive detection of cancer exosomes in clinical serum samples. This strategy showed high sensitivity and specificity, making it suitable for cancer diagnosis and biological studies.
Exosomes have attracted widespread interest due to their inherent advantages in tumor diagnosis and treatment monitoring. However, it is still a big challenge for highly sensitive and specific detection of exosome in real complexed samples. Herein, a molecular recognition triggered aptazyme cascade strategy was developed for ultrasensitive detection of cancer exosomes in clinical serum samples. In this design, one target exosome could capture a large quantity of aptazymes for the first-step signal amplification. And then the captured aptazyme was activated and recycled to release the fluorophore-labelled substrate strand for a cascaded signal amplification. Notably, the activation of aptazyme only occurs when it has bound with target exosome, ensuring a low background. The experimental results show that the limit of detection (LOD) and the limit of quantification (LOQ) are 3.5 x 10(3) particles/mu L and 1.7 x 10(4) particles/mu L, respectively, which is comparable to the results of most existed fluorescence-based exosome probes. Moreover, this assay possesses high specificity to distinguish exosomes derived from other cell lines. Furthermore, this fluorescence probe was utilized in cancer patient and healthy serum samples successfully, suggesting its great potential for clinical diagnosis and biological studies. (C) 2021 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
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