The evaluation of long term performance of microbial fuel cell based Pb toxicity shock sensor

Microbial fuel cell (MFC) sensor exhibits attractive prospects for online monitoring of water toxicity as an early warning device. However, the accumulation of dead cells in anode biofilm might decrease the sensing sensitivity of MFC during long term operation. In addition, with repeated exposure to toxins, the microbial community of anode biofilm would also adjust to build up higher endurance to environmental toxicity. In this study, the long term sensing sensitivity of MFC sensor and the microbial community changes were characterized with Pb2+ as the target toxin. The results show that newly formed biofilm with higher live/dead cell ratio exhibited higher sensitivity than mature biofilm. Modification of anodic biofilm via high current stimulation was applied to increase the ratio of live cells, which led to enhanced sensing sensitivity of MFC with mature anode biofilm. However, the enhancement was relatively limited for biofilm that was previously exposed to repeated Pb2+ shocks. Microbial community analysis revealed that the proportions of microbial species possessing higher environmental robustness, such as Hyphomicrobiaceae and Cloacibacillus, significantly increased in the anode biofilm after long term repeated Pb2+ shocks. (C) 2020 Elsevier Ltd. All rights reserved.

Automated summary

Microbial fuel cell (MFC) sensor has great potential for early warning water toxicity monitoring, but long-term operation may decrease its sensitivity due to dead cell accumulation in the anode biofilm. Exposing the biofilm to toxins repeatedly can lead to microbial community adjustment for higher endurance. Modification of the anodic biofilm can enhance sensitivity, especially for newly formed biofilm with higher live cell ratio.