Introduction of an Oxidation-responsive 4-Boronobenzyl Group into an Oligonucleotide through a Postmodification Approach

Oxidation-responsive oligonucleotides (ONs) can exhibit controlled functions by sensing dysregulated oxidation conditions in living systems. Herein, we describe a postmodification approach to constructing an oxidation-responsive ON that takes advantage of the reactivity of diazo compounds in introducing a 4-boronobenzyl group to a phosphate group at a 5'-terminal of an ON.

Automated summary

Oxidation-responsive oligonucleotides can exhibit controlled functions by sensing dysregulated oxidation conditions in living systems. The postmodification approach described here involves introducing a 4-boronobenzyl group to a phosphate group at the 5'-terminal of an ON using the reactivity of diazo compounds.