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Strategies for design of improved biocatalysts for industrial applications
Aravind Madhavan et al.
BIORESOURCE TECHNOLOGY (2017)
Photoactivatable Mussel-Based Underwater Adhesive Proteins by an Expanded Genetic Code
Matthias Hauf et al.
CHEMBIOCHEM (2017)
Deciphering the Fluorine Code-The Many Hats Fluorine Wears in a Protein Environment
Allison Ann Berger et al.
ACCOUNTS OF CHEMICAL RESEARCH (2017)
Inverse electron demand Diels-Alder reactions in chemical biology
B. L. Oliveira et al.
CHEMICAL SOCIETY REVIEWS (2017)
Biocatalysis with Unnatural Amino Acids: Enzymology Meets Xenobiology
Federica Agostini et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2017)
NHS-Esters As Versatile Reactivity-Based Probes for Mapping Proteome-Wide Ligandable Hotspots
Carl C. Ward et al.
ACS CHEMICAL BIOLOGY (2017)
Covalent Protein Labeling at Glutamic Acids
Pablo Martin-Gago et al.
CELL CHEMICAL BIOLOGY (2017)
Computational tools for enzyme improvement: why everyone can - and should - use them
Maximilian C. C. J. C. Ebert et al.
CURRENT OPINION IN CHEMICAL BIOLOGY (2017)
Irreversible Cysteine-Selective Protein Labeling Employing Modular Electrophilic Tetrafluoroethylation Reagents
Jiri Vaclavik et al.
CHEMISTRY-A EUROPEAN JOURNAL (2017)
Enzymatic site-selectivity enabled by structure-guided directed evolution
Jian-bo Wang et al.
CHEMICAL COMMUNICATIONS (2017)
Mechanistic Understanding of Lanthipeptide Biosynthetic Enzymes
Lindsay M. Repka et al.
CHEMICAL REVIEWS (2017)
Rational design of metalloenzymes: From single to multiple active sites
Ying-Wu Lin
COORDINATION CHEMISTRY REVIEWS (2017)
Transparent Window Vibrational Probes for the Characterization of Proteins With High Structural and Temporal Resolution
Ramkrishna Adhikary et al.
CHEMICAL REVIEWS (2017)
Physical and molecular bases of protein thermal stability and cold adaptation
Fabrizio Pucci et al.
CURRENT OPINION IN STRUCTURAL BIOLOGY (2017)
Bioconjugation of therapeutic proteins and enzymes using the expanded set of genetically encoded amino acids
Sung In Lim et al.
CRITICAL REVIEWS IN BIOTECHNOLOGY (2016)
Site-selective protein-modification chemistry for basic biology and drug development
Nikolaus Krall et al.
NATURE CHEMISTRY (2016)
Design of Catalytic Peptides and Proteins Through Rational and Combinatorial Approaches
Yoshiaki Maeda et al.
ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, VOL 18 (2016)
Effect of chemical modification of Novozym 435 on its performance in the alcoholysis of camelina oil
Carlos M. Verdasco-Martin et al.
BIOCHEMICAL ENGINEERING JOURNAL (2016)
Direct protein-protein conjugation by genetically introducing bioorthogonal functional groups into proteins
Sanggil Kim et al.
BIOORGANIC & MEDICINAL CHEMISTRY (2016)
Beyond the outer limits of nature by directed evolution
Patricia Molina-Espeja et al.
BIOTECHNOLOGY ADVANCES (2016)
PEGylation of cytochrome c at the level of lysine residues mediated by a microbial transglutaminase
Jian Qin Zhou et al.
BIOTECHNOLOGY LETTERS (2016)
Chemical Protein Modification through Cysteine
Smita B. Gunnoo et al.
CHEMBIOCHEM (2016)
The Life of Pi Star: Exploring the Exciting and Forbidden Worlds of the Benzophenone Photophore
Gyorgy Dorman et al.
CHEMICAL REVIEWS (2016)
Directed evolution and synthetic biology applications to microbial systems
Marcelo C. Bassalo et al.
CURRENT OPINION IN BIOTECHNOLOGY (2016)
How PEGylation influences protein conformational stability
Paul B. Lawrence et al.
CURRENT OPINION IN CHEMICAL BIOLOGY (2016)
Catalytic, kinetic and thermodynamic properties of Bacillus pumilus FH9 keratinase conjugated with activated pectin
Mohamed A. Abdel-Naby et al.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2016)
Genetically Encoded Photocrosslinkers for Identifying and Mapping Protein-Protein Interactions in Living Cells
Yi Yang et al.
IUBMB LIFE (2016)
Rational immobilization of lipase by combining the structure analysis and unnatural amino acid insertion
Anming Wang et al.
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2016)
Vibrational Stark Effects of Carbonyl Probes Applied to Reinterpret IR and Raman Data for Enzyme Inhibitors in Terms of Electric Fields at the Active Site
Samuel H. Schneider et al.
JOURNAL OF PHYSICAL CHEMISTRY B (2016)
Chemical modification of biocatalyst for function in supercritical CO2: In silico redesign of stable lipase
Hassan Monhemi et al.
JOURNAL OF SUPERCRITICAL FLUIDS (2016)
Solubilizing and Stabilizing Proteins in Anhydrous Ionic Liquids through Formation of Protein-Polymer Surfactant Nanoconstructs
Alex P. S. Brogans et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2016)
Transition Metal-Free Tryptophan-Selective Bioconjugation of Proteins
Yohei Seki et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2016)
A Critical Test of the Electrostatic Contribution to Catalysis with Noncanonical Amino Acids in Ketosteroid Isomerase
Yufan Wu et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2016)
Selenocysteine as a Latent Bioorthogonal Electrophilic Probe for Deubiquitylating Enzymes
Samuel D. Whedon et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2016)
A Chemically Programmed Proximal Ligand Enhances the Catalytic Properties of a Heme Enzyme
Anthony P. Green et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2016)
An Enzyme Cascade for Selective Modification of Tyrosine Residues in Structurally Diverse Peptides and Proteins
Anna-Winona Struck et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2016)
Chemoenzymatic Synthesis and Receptor Binding of Mannose-6-Phosphate (M6P)-Containing Glycoprotein Ligands Reveal Unusual Structural Requirements for M6P Receptor Recognition
Takahiro Yamaguchi et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2016)
The coming of age of de novo protein design
Po-Ssu Huang et al.
NATURE (2016)
Addicting diverse bacteria to a noncanonical amino acid
Drew S. Tack et al.
NATURE CHEMICAL BIOLOGY (2016)
Bioconjugation - using selective chemistry to enhance the properties of proteins and peptides as therapeutics and carriers
Smita B. Gunnoo et al.
ORGANIC & BIOMOLECULAR CHEMISTRY (2016)
Enhancing protein stability with extended disulfide bonds
Tao Liu et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2016)
Computational design of a homotrimeric metalloprotein with a trisbipyridyl core
Jeremy H. Mills et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2016)
Improved immobilization and stabilization of lipase from Rhizomucor miehei on octyl-glyoxyl agarose beads by using CaCl2
Laura Fernandez-Lopez et al.
PROCESS BIOCHEMISTRY (2016)
Design, synthesis, and testing toward a 57-codon genome
Nili Ostrov et al.
SCIENCE (2016)
Iminoboronates are efficient intermediates for selective, rapid and reversible N-terminal cysteine functionalisation
Helio Faustino et al.
CHEMICAL SCIENCE (2016)
At the Interface of Chemical and Biological Synthesis: An Expanded Genetic Code
Han Xiao et al.
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY (2016)
Genetically encoded FRET sensors using a fluorescent unnatural amino acid as a FRET donor
Wooseok Ko et al.
RSC ADVANCES (2016)
Towards Biocontained Cell Factories: An Evolutionarily Adapted Escherichia coli Strain Produces a New-to-nature Bioactive Lantibiotic Containing Thienopyrrole-Alanine
Anja Kuthning et al.
SCIENTIFIC REPORTS (2016)
Catalytic supramolecular self-assembled peptide nanostructures for ester hydrolysis
Gulcihan Gulseren et al.
JOURNAL OF MATERIALS CHEMISTRY B (2016)
Effect of modification of citraconic anhydrides on catalytic activity and thermostability of enzymes
Michiaki Matsumoto et al.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY (2016)
Controlled Orientation of Active Sites in a Nanostructured Multienzyme Complex
Sung In Lim et al.
SCIENTIFIC REPORTS (2016)
New Insights into the Biosynthetic Logic of Ribosomally Synthesized and Post-translationally Modified Peptide Natural Products
Manuel A. Ortega et al.
CELL CHEMICAL BIOLOGY (2016)
Measuring Electric Fields and Noncovalent Interactions Using the Vibrational Stark Effect
Stephen D. Fried et al.
ACCOUNTS OF CHEMICAL RESEARCH (2015)
Tyrosine-Specific Chemical Modification with in Situ Hemin-Activated Luminol Derivatives
Shinichi Sato et al.
ACS CHEMICAL BIOLOGY (2015)
Chemical Evolution of a Bacterial Proteome
Michael Georg Hoesl et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2015)
Expanding the Enzyme Universe: Accessing Non-Natural Reactions by Mechanism-Guided Directed Evolution
Hans Renata et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2015)
Manipulating the substrate specificity of murine dihydrofolate reductase enzyme using an expanded set of amino acids
Shun Zheng et al.
BIOCHEMICAL ENGINEERING JOURNAL (2015)
Cyanotryptophans as Novel Fluorescent Probes for Studying Protein Conformational Changes and DNA-Protein Interaction
Poulami Talukder et al.
BIOCHEMISTRY (2015)
Site-Specific Antibody-Drug Conjugates: The Nexus of Biciorthogonal Chemistry, Protein Engineering, and Drug Development
Paresh Agarwal et al.
BIOCONJUGATE CHEMISTRY (2015)
An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes
Nur Royhaila Mohamad et al.
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT (2015)
Incorporating unnatural amino acids to engineer biocatalysts for industrial bioprocess applications
Yuvaraj Ravikumar et al.
BIOTECHNOLOGY JOURNAL (2015)
Orthogonal Translation Meets Electron Transfer: In Vivo Labeling of Cytochrome c for Probing Local Electric Fields
Jan Voeller et al.
CHEMBIOCHEM (2015)
Advances in Chemical Protein Modification
Omar Boutureira et al.
CHEMICAL REVIEWS (2015)
Developments and recent advancements in the field of endogenous amino acid selective bond forming reactions for bioconjugation
Oleksandr Koniev et al.
CHEMICAL SOCIETY REVIEWS (2015)
Chemical Modification of Lipase for Rational Enhancement of Enantioselectivity
Tadashi Ema et al.
CHEMISTRY LETTERS (2015)
Covalent protein modification: the current landscape of residue-specific electrophiles
D. Alexander Shannon et al.
CURRENT OPINION IN CHEMICAL BIOLOGY (2015)
Metallopeptide catalysts and artificial metalloenzymes containing unnatural amino acids
Jared C. Lewis
CURRENT OPINION IN CHEMICAL BIOLOGY (2015)
Improving Catalytic Performance of Candida rugosa Lipase by Chemical Modification with Polyethylene Glycol Functional Ionic Liquids
Xiujuan Li et al.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH (2015)
Nature and strength of sulfur-centred hydrogen bonds: laser spectroscopic investigations in the gas phase and quantum-chemical calculations
Himansu S. Biswal et al.
INTERNATIONAL REVIEWS IN PHYSICAL CHEMISTRY (2015)
Enhanced Enzyme Stability Through Site-Directed Covalent Immobilization
Jeffrey Chun Yu Wu et al.
JOURNAL OF BIOTECHNOLOGY (2015)
Chemical amination of Rhizopus oryzae lipase for multipoint covalent immobilization on epoxy-functionalized supports: Modulation of stability and selectivity
Maryam Ashjari et al.
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2015)
Defining the Role of Tyrosine and Rational Tuning of Oxidase Activity by Genetic Incorporation of Unnatural Tyrosine Analogs
Yang Yu et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2015)
New Tricks for Old Proteins: Single Mutations in a Nonenzymatic Protein Give Rise to Various Enzymatic Activities
Yurii S. Moroz et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2015)
Supramolecular Assembly of Artificial Metalloenzymes Based on the Dimeric Protein LmrR as Promiscuous Scaffold
Jeffrey Bos et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2015)
Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids
Miriam Amiram et al.
NATURE BIOTECHNOLOGY (2015)
Efficient genetic encoding of phosphoserine and its nonhydrolyzable analog
Daniel T. Rogerson et al.
NATURE CHEMICAL BIOLOGY (2015)
Copper-catalysed azide-alkyne cycloadditions (CuAAC): an update
Estela Haldon et al.
ORGANIC & BIOMOLECULAR CHEMISTRY (2015)
In-Frame Amber Stop Codon Replacement Mutagenesis for the Directed Evolution of Proteins Containing Non-Canonical Amino Acids: Identification of Residues Open to Bio-Orthogonal Modification
James A. J. Arpino et al.
PLOS ONE (2015)
Exploring the potential impact of an expanded genetic code on protein function
Han Xiao et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)
Unnatural amino acid mutagenesis-based enzyme engineering
Yuvaraj Ravikumar et al.
TRENDS IN BIOTECHNOLOGY (2015)
Significant improvement of oxidase activity through the genetic incorporation of a redox-active unnatural amino acid
Yang Yu et al.
CHEMICAL SCIENCE (2015)
Designing logical codon reassignment - Expanding the chemistry in biology
Anaelle Dumas et al.
CHEMICAL SCIENCE (2015)
Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids
Ivana Drienovska et al.
CHEMICAL SCIENCE (2015)
Engineering a dirhodium artificial metalloenzyme for selective olefin cyclopropanation
Poonam Srivastava et al.
NATURE COMMUNICATIONS (2015)
Enantioselectivity and catalysis improvements of Pseudomonas cepacia lipase with Tyr and Asp modification
Jing Li et al.
CATALYSIS SCIENCE & TECHNOLOGY (2015)
Highly reproductive Escherichia coli cells with no specific assignment to the UAG codon
Takahito Mukai et al.
SCIENTIFIC REPORTS (2015)
Protein stabilization utilizing a redefined codon
Kazumasa Ohtake et al.
SCIENTIFIC REPORTS (2015)
Incorporating unnatural amino acids to engineer biocatalysts for industrial bioprocess applications
Yuvaraj Ravikumar et al.
BIOTECHNOLOGY JOURNAL (2015)
Biosynthesis of a Novel Glutamate Racemase Containing a Site-Specific 7-Hydroxycoumarin Amino Acid: Enzyme-Ligand Promiscuity Revealed at the Atomistic Level
Sondra F. Dean et al.
ACS CENTRAL SCIENCE (2015)
Engineering Transaminase for Stability Enhancement and Site-Specific Immobilization through Multiple Noncanonical Amino Acids Incorporation
Kanagavel Deepankumar et al.
CHEMCATCHEM (2015)
Using Unnatural Amino Acid Mutagenesis To Probe the Regulation of PRMT1
Heather L. Rust et al.
ACS CHEMICAL BIOLOGY (2014)
Unnatural Amino Acid Mutagenesis Reveals Dimerization As a Negative Regulatory Mechanism of VHR's Phosphatase Activity
Karolina Pavic et al.
ACS CHEMICAL BIOLOGY (2014)
Genetically Encoding an Electrophilic Amino Acid for Protein Stapling and Covalent Binding to Native Receptors
Xiao-Hua Chen et al.
ACS CHEMICAL BIOLOGY (2014)
Enhancing Thermostability and Organic Solvent Tolerance of w-Transaminase through Global Incorporation of Fluorotyrosine
Kanagavel Deepankumar et al.
ADVANCED SYNTHESIS & CATALYSIS (2014)
Recoding the Genetic Code with Selenocysteine
Markus J. Broecker et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2014)
Proximity-Enabled Protein Crosslinking through Genetically Encoding Haloalkane Unnatural Amino Acids
Zheng Xiang et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2014)
Modification of Lysine Residues of Horseradish Peroxidase and Its Effect on Stability and Structure of the Enzyme
Leila Hassani et al.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2014)
Mapping a Ketosynthase:Acyl Carrier Protein Binding Interface via Unnatural Amino Acid-Mediated Photo-Cross-Linking
Zhixia Ye et al.
BIOCHEMISTRY (2014)
Methionine oxidation and reduction in proteins
Geumsoo Kim et al.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS (2014)
Charge Engineering of Cellulases Improves Ionic Liquid Tolerance and Reduces Lignin Inhibition
Erik M. Nordwald et al.
BIOTECHNOLOGY AND BIOENGINEERING (2014)
Comparative genomics highlights the unique biology of Methanomassiliicoccales, a Thermoplasmatales-related seventh order of methanogenic archaea that encodes pyrrolysine
Guillaume Borrel et al.
BMC GENOMICS (2014)
A General Method for Artificial Metalloenzyme Formation through Strain-Promoted Azide-Alkyne Cycloaddition
Hao Yang et al.
CHEMBIOCHEM (2014)
Modulating the pKa of a Tyrosine in KlenTaq DNA Polymerase that Is Crucial for Abasic Site Bypass by in Vivo Incorporation of a Non-canonical Amino Acid
Nina Blatter et al.
CHEMBIOCHEM (2014)
Enhancing the Efficiency and Regioselectivity of P450 Oxidation Catalysts by Unnatural Amino Acid Mutagenesis
Joshua N. Kolev et al.
CHEMBIOCHEM (2014)
Cell Surface Display Yields Evolvable, Clickable Antibody Fragments
James A. Van Deventer et al.
CHEMBIOCHEM (2014)
A Bacterial Strain with a Unique Quadruplet Codon Specifying Non-native Amino Acids
Abhishek Chatterjee et al.
CHEMBIOCHEM (2014)
Protein stapling via azide-alkyne ligation
Diya M. Abdeljabbar et al.
CHEMICAL COMMUNICATIONS (2014)
Cellular Incorporation of Unnatural Amino Acids and Bioorthogonal Labeling of Proteins
Kathrin Lang et al.
CHEMICAL REVIEWS (2014)
Protein Design: Toward Functional Metalloenzymes
Fangting Yu et al.
CHEMICAL REVIEWS (2014)
Evolution of fluorinated enzymes: An emerging trend for biocatalyst stabilization
Hernan Biava et al.
ENGINEERING IN LIFE SCIENCES (2014)
Effects of chemical modification by chitooligosaccharide on enzyme activity and stability of yeast β-D-fructofuranosidase
Chunyi Li
ENZYME AND MICROBIAL TECHNOLOGY (2014)
Efficient improvement on stability of sarcosine oxidase via poly-lysine modification on enzyme surface
Yanjun Tong et al.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2014)
Coupling Bioorthogonal Chemistries with Artificial Metabolism: Intracellular Biosynthesis of Azidohomoalanine and Its Incorporation into Recombinant Proteins
Ying Ma et al.
MOLECULES (2014)
Design of activated serine-containing catalytic triads with atomic-level accuracy
Sridharan Rajagopalan et al.
NATURE CHEMICAL BIOLOGY (2014)
Bacterial methionine biosynthesis
Matteo P. Ferla et al.
MICROBIOLOGY-SGM (2014)
Cell-free unnatural amino acid incorporation with alternative energy systems and linear expression templates
Prashanta Shrestha et al.
NEW BIOTECHNOLOGY (2014)
A novel intermediate in transcription initiation by human mitochondrial RNA polymerase
Yaroslav I. Morozov et al.
NUCLEIC ACIDS RESEARCH (2014)
Site-Specific Bioconjugation of a Murine Dihydrofolate Reductase Enzyme by Copper(I)-Catalyzed Azide-Alkyne Cycloaddition with Retained Activity
Sung In Lim et al.
PLOS ONE (2014)
The Gate Keeper'' Role of Trp222 Determines the Enantiopreference of Diketoreductase toward 2-Chloro-1-Phenylethanone
Hairong Ma et al.
PLOS ONE (2014)
Diverse Functionalization of Aurora-A Kinase at Specified Surface and Buried Sites by Native Chemical Modification
Fiona Rowan et al.
PLOS ONE (2014)
CHEMICAL MODIFICATION OF L-ASPARAGINASE FROM Cladosporium sp FOR IMPROVED ACTIVITY AND THERMAL STABILITY
N. S. Mohan Kumar et al.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY (2014)
Measurement of histidine pKa values and tautomer populations in invisible protein states
Alexandar L. Hansen et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2014)
Tuning of Lecitase features via solid-phase chemical modification: Effect of the immobilization protocol
Cristina Garcia-Galan et al.
PROCESS BIOCHEMISTRY (2014)
Improving the catalytic performance of porcine pancreatic lipase in the presence of [MMIm][MeSO4] with the modification of functional ionic liquids
Ru Jia et al.
PROCESS BIOCHEMISTRY (2014)
Extreme electric fields power catalysis in the active site of ketosteroid isomerase
Stephen D. Fried et al.
SCIENCE (2014)
Functional tuning and expanding of myoglobin by rational protein design
Lin YingWu et al.
SCIENCE CHINA-CHEMISTRY (2014)
A catalytic role for methionine revealed by a combination of computation and experiments on phosphite dehydrogenase
Kara E. Ranaghan et al.
CHEMICAL SCIENCE (2014)
Enzyme activity in liquid lipase melts as a step towards solvent-free biology at 150 °C
Alex P. S. Brogan et al.
NATURE COMMUNICATIONS (2014)
Selective chemical protein modification
Christopher D. Spicer et al.
NATURE COMMUNICATIONS (2014)
Amination of enzymes to improve biocatalyst performance: coupling genetic modification and physicochemical tools
Rafael C. Rodrigues et al.
RSC ADVANCES (2014)
Unnatural amino acid incorporation in E. coli: current and future applications in the design of therapeutic proteins
Kim Wals et al.
FRONTIERS IN CHEMISTRY (2014)
Reversible, Long-Range Radical Transfer in E-coli Class la Ribonucleotide Reductase
Ellen C. Minnihan et al.
ACCOUNTS OF CHEMICAL RESEARCH (2013)
Computational Design of Enone-Binding Proteins with Catalytic Activity for the Morita-Baylis-Hillman Reaction
Sinisa Bjelic et al.
ACS CHEMICAL BIOLOGY (2013)
Probing the Function of the Tyr-Cys Cross-Link in Metalloenzymes by the Genetic Incorporation of 3-Methylthiotyrosine
Qing Zhou et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2013)
Computational Enzyme Design
Gert Kiss et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2013)
Fluorescent Biphenyl Derivatives of Phenylalanine Suitable for Protein Modification
Shengxi Chen et al.
BIOCHEMISTRY (2013)
A Versatile Platform for Single- and Multiple-Unnatural Amino Acid Mutagenesis in Escherichia coli
Abhishek Chatterjee et al.
BIOCHEMISTRY (2013)
Protein Engineering For Directed Immobilization
Erik Steen Redeker et al.
BIOCONJUGATE CHEMISTRY (2013)
Controlling Enzyme Inhibition Using an Expanded Set of Genetically Encoded Amino Acids
Shun Zheng et al.
BIOTECHNOLOGY AND BIOENGINEERING (2013)
Stabilization of Enzymes in Ionic Liquids Via Modification of Enzyme Charge
Erik M. Nordwald et al.
BIOTECHNOLOGY AND BIOENGINEERING (2013)
Structural Insights into the Recovery of Aldolase Activity in N-Acetylneuraminic Acid Lyase by Replacement of the Catalytically Active Lysine with γ-Thialysine by Using a Chemical Mutagenesis Strategy
Nicole Timms et al.
CHEMBIOCHEM (2013)
Aligning an endoglucanase Cel5A from Thermobifida fusca on a DNA scaffold: potent design of an artificial cellulosome
Yutaro Mori et al.
CHEMICAL COMMUNICATIONS (2013)
Cation-π Interaction: Its Role and Relevance in Chemistry, Biology, and Material Science
A. Subha Mahadevi et al.
CHEMICAL REVIEWS (2013)
19F NMR: a valuable tool for studying biological events
Han Chen et al.
CHEMICAL SOCIETY REVIEWS (2013)
Protein engineering with unnatural amino acids
William H. Zhang et al.
CURRENT OPINION IN STRUCTURAL BIOLOGY (2013)
Chemical approaches for the construction of multi-enzyme reaction systems
Sanne Schoffelen et al.
CURRENT OPINION IN STRUCTURAL BIOLOGY (2013)
Activity analysis of the carbodiimide-mediated amine coupling reaction on self-assembled monolayers by cyclic voltammetry
Lin Liu et al.
ELECTROCHIMICA ACTA (2013)
Catalytic activity and thermostability of enzymes immobilized on silanized surface: Influence of the crosslinking agent
Nesrine Aissaoui et al.
ENZYME AND MICROBIAL TECHNOLOGY (2013)
Chemical modification of wheat β-amylase by trinitrobenzenesulfonic acid, methoxypolyethylene glycol, and glutaraldehyde to improve its thermal stability and activity
Tadessa Daba et al.
ENZYME AND MICROBIAL TECHNOLOGY (2013)
Tailing DNA aptamers with a functional protein by two-step enzymatic reaction
Mari Takahara et al.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2013)
Calculation of Vibrational Shifts of Nitrile Probes in the Active Site of Ketosteroid Isomerase upon Ligand Binding
Joshua P. Layfield et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)
Detection of Dihydrofolate Reductase Conformational Change by FRET Using Two Fluorescent Amino Acids
Shengxi Chen et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)
Computational Design of an Unnatural Amino Acid Dependent Metalloprotein with Atomic Level Accuracy
Jeremy H. Mills et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)
An Introduction to Biological NMR Spectroscopy
Dominique Marion
MOLECULAR & CELLULAR PROTEOMICS (2013)
Expanding the chemistry of fluorescent protein biosensors through genetic incorporation of unnatural amino acids
Wei Niu et al.
MOLECULAR BIOSYSTEMS (2013)
Non-Natural Amino Acids for Protein Engineering and New Protein Chemistries
Inchan Kwon et al.
MACROMOLECULAR CHEMISTRY AND PHYSICS (2013)
Adding an unnatural covalent bond to proteins through proximity-enhanced bioreactivity
Zheng Xiang et al.
NATURE METHODS (2013)
Directed evolution as a powerful synthetic biology tool
Ryan E. Cobb et al.
METHODS (2013)
Chemical modification for improving activity and stability of lipase B from Candida antarctica with imidazolium-functional ionic liquids
Ru Jia et al.
ORGANIC & BIOMOLECULAR CHEMISTRY (2013)
Bioorthogonal labelling of biomolecules: new functional handles and ligation methods
Marjoke F. Debets et al.
ORGANIC & BIOMOLECULAR CHEMISTRY (2013)
Enhancing Catalytic Performance of Porcine Pancreatic Lipase by Covalent Modification Using Functional Ionic Liquids
Ru Jia et al.
ACS CATALYSIS (2013)
Artificial Metalloenzymes and Metallopeptide Catalysts for Organic Synthesis
Jared C. Lewis
ACS CATALYSIS (2013)
Non-canonical amino acids as a useful synthetic biological tool for lipase-catalysed reactions in hostile environments
Carlos G. Acevedo-Rocha et al.
CATALYSIS SCIENCE & TECHNOLOGY (2013)
Chemistry for peptide and protein PEGylation
M. J. Roberts et al.
ADVANCED DRUG DELIVERY REVIEWS (2012)
Biochemical analysis with the expanded genetic lexicon
Hui-wang Ai
ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2012)
Significant Increase of Oxidase Activity through the Genetic Incorporation of a Tyrosine-Histidine Cross-Link in a Myoglobin Model of Heme-Copper Oxidase
Xiaohong Liu et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2012)
The Effect of Chemical Modification with Pyromellitic Anhydride on Structure, Function, and Thermal Stability of Horseradish Peroxidase
Leila Hassani
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2012)
Altering the Interfacial Activation Mechanism of a Lipase by Solid-Phase Selective Chemical Modification
Fernando Lopez-Gallego et al.
BIOCHEMISTRY (2012)
Site-Selective Lysine Modification of Native Proteins and Peptides via Kinetically Controlled Labeling
Xi Chen et al.
BIOCONJUGATE CHEMISTRY (2012)
Sulfonyl Fluoride Analogues as Activity-Based Probes for Serine Proteases
D. Alexander Shannon et al.
CHEMBIOCHEM (2012)
Fluorinated amino acids: compatibility with native protein structures and effects on protein-protein interactions
Mario Salwiczek et al.
CHEMICAL SOCIETY REVIEWS (2012)
Recent advances in genetic code engineering in Escherichia coli
Michael Georg Hoesl et al.
CURRENT OPINION IN BIOTECHNOLOGY (2012)
Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria
Zhengxu Gao et al.
ENERGY & ENVIRONMENTAL SCIENCE (2012)
Enhanced phosphoserine insertion during Escherichia coli protein synthesis via partial UAG codon reassignment and release factor 1 deletion
Ilka U. Heinemann et al.
FEBS LETTERS (2012)
Near-cognate suppression of amber, opal and quadruplet codons competes with aminoacyl-tRNAPyl for genetic code expansion
Patrick O'Donoghue et al.
FEBS LETTERS (2012)
Chemical modification of Horseradish peroxidase with carboxylic anhydrides: Effect of negative charge and hydrophilicity of the modifiers on thermal stability
Leila Hassani
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2012)
Computational Design of Catalytic Dyads and Oxyanion Holes for Ester Hydrolysis
Florian Richter et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2012)
Control of Enzyme-Solid Interactions via Chemical Modification
Ruma Chowdhury et al.
LANGMUIR (2012)
The structures of type I polyketide synthases
Adrian T. Keatinge-Clay
NATURAL PRODUCT REPORTS (2012)
Designer proteins: applications of genetic code expansion in cell biology
Lloyd Davis et al.
NATURE REVIEWS MOLECULAR CELL BIOLOGY (2012)
How ionic liquids can help to stabilize native proteins
Hermann Weingaertner et al.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2012)
Iterative approach to computational enzyme design
Heidi K. Privett et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2012)
Chemical amination of lipase B from Candida antarctica is an efficient solution for the preparation of crosslinked enzyme aggregates
Magaly Galvis et al.
PROCESS BIOCHEMISTRY (2012)
Hyper-thermal stability and unprecedented re-folding of solvent-free liquid myoglobin
Alex P. S. Brogan et al.
CHEMICAL SCIENCE (2012)
Structural insights into the potential of 4-fluoroproline to modulate biophysical properties of proteins
Bastian Holzberger et al.
CHEMICAL SCIENCE (2012)
ω-Transaminases for the Production of Optically Pure Amines and Unnatural Amino Acids
Sam Mathew et al.
ACS CATALYSIS (2012)
Different strategies to enhance the activity of lipase catalysts
Marzia Marciello et al.
CATALYSIS SCIENCE & TECHNOLOGY (2012)
Artificial Metalloenzymes Based on the Biotin-Avidin Technology: Enantioselective Catalysis and Beyond
Thomas R. Ward
ACCOUNTS OF CHEMICAL RESEARCH (2011)
A Tag-and-Modify Approach to Site-Selective Protein Modification
Justin M. Chalker et al.
ACCOUNTS OF CHEMICAL RESEARCH (2011)
Multi-Enzymatic Cascade Reactions: Overview and Perspectives
Emanuele Ricca et al.
ADVANCED SYNTHESIS & CATALYSIS (2011)
Development of a Selective, Sensitive, and Reversible Biosensor by the Genetic Incorporation of a Metal-Binding Site into Green Fluorescent Protein
Niraikulam Ayyadurai et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2011)
Light-Activated Gene Editing with a Photocaged Zinc-Finger Nuclease
Chungjung Chou et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2011)
Characteristic features and biotechnological applications of cross-linked enzyme aggregates (CLEAs)
Roger A. Sheldon
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2011)
Bioconjugation of L-3,4-Dihydroxyphenylalanine Containing Protein with a Polysaccharide
Niraikulam Ayyadurai et al.
BIOCONJUGATE CHEMISTRY (2011)
Tunable Degradation of Maleimide-Thiol Adducts in Reducing Environments
Aaron D. Baldwin et al.
BIOCONJUGATE CHEMISTRY (2011)
A chemically modified lipase preparation for catalyzing the transesterification reaction in even highly polar organic solvents
Kusum Solanki et al.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS (2011)
Expanding and Engineering the Genetic Code in a Single Expression Experiment
Michael G. Hoesl et al.
CHEMBIOCHEM (2011)
Enhanced Refoldability and Thermoactivity of Fluorinated Phosphotriesterase
Peter James Baker et al.
CHEMBIOCHEM (2011)
Lipase Congeners Designed by Genetic Code Engineering
Michael G. Hoesl et al.
CHEMCATCHEM (2011)
Ionic Liquid-Inspired Cations Covalently Bound to Formate Dehydrogenase Improve its Stability and Activity in Ionic Liquids
Mourad Bekhouche et al.
CHEMCATCHEM (2011)
Enzyme Surface Glycosylation in the Solid Phase: Improved Activity and Selectivity of Candida Antarctica Lipase B
Melissa L. E. Gutarra et al.
CHEMCATCHEM (2011)
Direct radiolabelling of proteins at cysteine using [F-18]-fluorosugars
Omar Boutureira et al.
CHEMICAL COMMUNICATIONS (2011)
Chemical modification in the creation of novel biocatalysts
Alba Diaz-Rodriguez et al.
CURRENT OPINION IN CHEMICAL BIOLOGY (2011)
Optimizing non-natural protein function with directed evolution
Eric M. Brustad et al.
CURRENT OPINION IN CHEMICAL BIOLOGY (2011)
Advances in the mechanism and understanding of site-selective noncanonical amino acid incorporation
Alicja K. Antonczak et al.
CURRENT OPINION IN STRUCTURAL BIOLOGY (2011)
Buffer-mediated activation of Candida antarctica lipase B dissolved in hydroxyl-functionalized ionic liquids
Guangnan Ou et al.
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2011)
Two-Dimensional IR Spectroscopy of Protein Dynamics Using Two Vibrational Labels: A Site-Specific Genetically Encoded Unnatural Amino Acid and an Active Site Ligand
Megan C. Thielges et al.
JOURNAL OF PHYSICAL CHEMISTRY B (2011)
Synthetic Approach to Stop-Codon Scanning Mutagenesis
Lihua Nie et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2011)
Improving a Natural Enzyme Activity through Incorporation of Unnatural Amino Acids
Isaac N. Ugwumba et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2011)
Incorporation of Fluorotyrosines into Ribonucleotide Reductase Using an Evolved, Polyspecific Aminoacyl-tRNA Synthetase
Ellen C. Minnihan et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2011)
Probing Eudesmane Cation-π Interactions in Catalysis by Aristolochene Synthase with Non-canonical Amino Acids
Juan A. Faraldos et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2011)
Kinetics of Radical Intermediate Formation and Deoxynucleotide Production in 3-Aminotyrosine-Substituted Escherichia coli Ribonucleotide Reductases
Ellen C. Minnihan et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2011)
Modulating substrate specificity of histone acetyltransferase with unnatural amino acids
Kinjal Rajesh Mehta et al.
MOLECULAR BIOSYSTEMS (2011)
A photocrosslinking assay for reporting protein interactions in polyketide and fatty acid synthases
Zhixia Ye et al.
MOLECULAR BIOSYSTEMS (2011)
Choosing an effective protein bioconjugation strategy
Nicholas Stephanopoulos et al.
NATURE CHEMICAL BIOLOGY (2011)
Flexizymes for genetic code reprogramming
Yuki Goto et al.
NATURE PROTOCOLS (2011)
Structural interconversions modulate activity of Escherichia coli ribonucleotide reductase
Nozomi Ando et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2011)
Intramolecular isopeptide bonds: protein crosslinks built for stress?
Hae Joo Kang et al.
TRENDS IN BIOCHEMICAL SCIENCES (2011)
Asymmetric Allosteric Signaling in Aspartate Transcarbamoylase
Kimberly R. Mendes et al.
ACS CHEMICAL BIOLOGY (2010)
Selenium incorporation using recombinant techniques
Helen Walden
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY (2010)
A Highly Active DNA Polymerase with a Fluorous Core
Bastian Holzberger et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2010)
In Vivo Double and Triple Labeling of Proteins Using Synthetic Amino Acids
Sandra Lepthien et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2010)
Adding New Chemistries to the Genetic Code
Chang C. Liu et al.
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 79 (2010)
Photocaged T7 RNA Polymerase for the Light Activation of Transcription and Gene Function in Pro- and Eukaryotic Cells
Chungjung Chou et al.
CHEMBIOCHEM (2010)
Parallel Incorporation of Different Fluorinated Amino Acids: On the Way to Teflon Proteins
Lars Merkel et al.
CHEMBIOCHEM (2010)
Aza-dibenzocyclooctynes for fast and efficient enzyme PEGylation via copper-free (3+2) cycloaddition
Marjoke F. Debets et al.
CHEMICAL COMMUNICATIONS (2010)
Fluorescent Analogs of Biomolecular Building Blocks: Design, Properties, and Applications
Renatus W. Sinkeldam et al.
CHEMICAL REVIEWS (2010)
Proton-Coupled Electron Flow in Protein Redox Machines
Jillian L. Dempsey et al.
CHEMICAL REVIEWS (2010)
Protein Chemical Modification on Endogenous Amino Acids
Emmanuel Basle et al.
CHEMISTRY & BIOLOGY (2010)
Beyond directed evolution-semi-rational protein engineering and design
Stefan Lutz
CURRENT OPINION IN BIOTECHNOLOGY (2010)
Residue-specific incorporation of non-canonical amino acids into proteins: recent developments and applications
Jeremiah A. Johnson et al.
CURRENT OPINION IN CHEMICAL BIOLOGY (2010)
Chemoenzymatic methods for site-specific protein modification
David Rabuka
CURRENT OPINION IN CHEMICAL BIOLOGY (2010)
Protein engineering of microbial enzymes
Dominique Boettcher et al.
CURRENT OPINION IN MICROBIOLOGY (2010)
Two-dimensional NMR and All-atom Molecular Dynamics of Cytochrome P450 CYP119 Reveal Hidden Conformational Substates
Jed N. Lampe et al.
JOURNAL OF BIOLOGICAL CHEMISTRY (2010)
Beyond the Canonical 20 Amino Acids: Expanding the Genetic Lexicon
Travis S. Young et al.
JOURNAL OF BIOLOGICAL CHEMISTRY (2010)
Site-specific labeling of proteins with NMR-active unnatural amino acids
David H. Jones et al.
JOURNAL OF BIOMOLECULAR NMR (2010)
An Enhanced System for Unnatural Amino Acid Mutagenesis in E. coli
Travis S. Young et al.
JOURNAL OF MOLECULAR BIOLOGY (2010)
Chemical modification of stem bromelain with anhydride groups to enhance its stability and catalytic activity
Yong Xue et al.
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2010)
Chemical modifications by ionic liquid-inspired cations improve the activity and the stability of formate dehydrogenase in [MMIm][Me2PO4]
Mourad Bekhouche et al.
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2010)
Site-Specific Incorporation of 3-Nitrotyrosine as a Probe of pKa Perturbation of Redox-Active Tyrosines in Ribonucleotide Reductase
Kenichi Yokoyama et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2010)
Replacing 32 Proline Residues by a Noncanonical Amino Acid Results in a Highly Active DNA Polymerase
Bastian Holzberger et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2010)
Protein Modification, Bioconjugation, and Disulfide Bridging Using Bromomaleimides
Mark E. B. Smith et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2010)
Protein 19F NMR in Escherichia coli
Conggang Li et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2010)
Incorporation of unnatural amino acids for synthetic biology
Natalya Voloshchuk et al.
MOLECULAR BIOSYSTEMS (2010)
Residue-specific global fluorination of Candida antarctica lipase B in Pichia pastoris
Nediljko Budisa et al.
MOLECULAR BIOSYSTEMS (2010)
Reversible dioxygen binding in solvent-free liquid myoglobin
Adam W. Perriman et al.
NATURE CHEMISTRY (2010)
Activation and stabilization of enzymes in ionic liquids
Muhammad Moniruzzaman et al.
ORGANIC & BIOMOLECULAR CHEMISTRY (2010)
Superfamily-wide portrait of serine hydrolase inhibition achieved by library-versus-library screening
Daniel A. Bachovchin et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2010)
Roles of glutamates and metal ions in a rationally designed nitric oxide reductase based on myoglobin
Ying-Wu Lin et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2010)
Enhanced activity of an immobilized lipase promoted by site-directed chemical modification with polymers
Cesar A. Godoy et al.
PROCESS BIOCHEMISTRY (2010)
Expansion of the genetic code: Site-directed p-fluoro-phenylalanine incorporation in Escherichia coli
Rolf Furter
PROTEIN SCIENCE (2010)
A General Method for Scanning Unnatural Amino Acid Mutagenesis
Kelly A. Daggett et al.
ACS CHEMICAL BIOLOGY (2009)
A Light-Activated DNA Polymerase
Chungjung Chou et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2009)
Solvent-Free Protein Liquids and Liquid Crystals
Adam W. Perriman et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2009)
Efforts Toward the Direct Experimental Characterization of Enzyme Microenvironments: Tyrosine100 in Dihydrofolate Reductase
Dan Groff et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2009)
Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality
Ellen M. Sletten et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2009)
Proton-Coupled Electron Transfer in Biology: Results from Synergistic Studies in Natural and Model Systems
Steven Y. Reece et al.
ANNUAL REVIEW OF BIOCHEMISTRY (2009)
Multiply mutated Gaussia luciferases provide prolonged and intense bioluminescence
John P. Welsh et al.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2009)
Probing Protein Folding Using Site-Specifically Encoded Unnatural Amino Acids as FRET Donors with Tryptophan
Shigeki J. Miyake-Stoner et al.
BIOCHEMISTRY (2009)
The pH-Dependent Formation of PEGylated Bovine Lactoferrin by Branched Polyethylene Glycol (PEG)-N-Hydroxysuccinimide (NHS) Active Esters
Yasuhiro Nojima et al.
BIOLOGICAL & PHARMACEUTICAL BULLETIN (2009)
Synthesis and evaluation of a cyclic imine derivative conjugated to a fluorescent molecule for labeling of proteins
Hai-Ming Guo et al.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS (2009)
Glycoprotein Synthesis: An Update
David P. Gamblin et al.
CHEMICAL REVIEWS (2009)
Expanding the Genetic Code for Biological Studies
Qian Wang et al.
CHEMISTRY & BIOLOGY (2009)
Electron-Deficient Alkynes as Cleavable Reagents for the Modification of Cysteine-Containing Peptides in Aqueous Medium
Hoi-Yan Shiu et al.
CHEMISTRY-A EUROPEAN JOURNAL (2009)
Protein Cysteine Modifications: (2) Reactivity Specificity and Topics of Medicinal Chemistry and Protein Engineering
N. Nagahara et al.
CURRENT MEDICINAL CHEMISTRY (2009)
Chemical cross-linking with NHS esters: a systematic study on amino acid reactivities
Stefanie Maedler et al.
JOURNAL OF MASS SPECTROMETRY (2009)
Genetic Incorporation of a Metal-Ion Chelating Amino Acid into Proteins as a Biophysical Probe
Hyun Soo Lee et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2009)
Expanded chemical diversity sampling through whole protein evolution
Amy J. Baldwin et al.
MOLECULAR BIOSYSTEMS (2009)
Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells
Jeffrey K. Takimoto et al.
MOLECULAR BIOSYSTEMS (2009)
Directed evolution drives the next generation of biocatalysts
Nicholas J. Turner
NATURE CHEMICAL BIOLOGY (2009)
Design of anti- and pro-aggregation variants to assess the effects of methionine oxidation in human prion protein
Christina Wolschner et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2009)
Immobilization-stabilization of the lipase from Thermomyces lanuginosus: Critical role of chemical amination
Rafael C. Rodrigues et al.
PROCESS BIOCHEMISTRY (2009)
Activity-based protein profiling: From enzyme chemistry
Benjamin F. Cravatt et al.
ANNUAL REVIEW OF BIOCHEMISTRY (2008)
The electronic structure of the Cys-Tyr• free radical in galactose oxidase determined by EPR spectroscopy
Yuk-Ki Lee et al.
BIOCHEMISTRY (2008)
Attachment of peptide building blocks to proteins through tyrosine bioconjugation
Dante W. Romanini et al.
BIOCONJUGATE CHEMISTRY (2008)
Contribution of linker stability to the activities of anticancer immunoconjugates
Stephen C. Alley et al.
BIOCONJUGATE CHEMISTRY (2008)
Glycation improves the thermostability of trypsin and chymotrypsin
Van Thong Pham et al.
BIOTECHNOLOGY AND BIOENGINEERING (2008)
One heme, diverse functions: Using biosynthetic myoglobin models to gain insights into heme-copper oxidases and nitric oxide reductases
Natasha Yeung et al.
CHEMISTRY & BIODIVERSITY (2008)
Affinity-labeling-based introduction of a reactive handle for natural protein modification
Haruto Wakabayashi et al.
CHEMISTRY-AN ASIAN JOURNAL (2008)
Ligand-Induced Conformational Heterogeneity of Cytochrome P450 CYP119 Identified by 2D NMR Spectroscopy with the Unnatural Amino Acid 13C-p-Methoxyphenylalanine
Jed N. Lampe et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2008)
Characterization of a three-component coupling reaction on proteins by isotopic labeling and nuclear magnetic resonance spectroscopy
Jesse M. McFarland et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2008)
Biosynthesis of a site-specific DNA cleaving protein
Hyun Soo Lee et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2008)
In vivo incorporation of unnatural amino acids to probe structure, dynamics, and ligand binding in a large protein by nuclear magnetic resonance spectroscopy
Susan E. Cellitti et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2008)
Facile conversion of cysteine and alkyl cysteines to dehydroalanine on protein surfaces: Versatile and switchable access to functionalized proteins
Goncalo J. L. Bernardes et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2008)
Kemp elimination catalysts by computational enzyme design
Daniela Rothlisberger et al.
NATURE (2008)
Expanded molecular diversity generation during directed evolution by trinucleotide exchange (TriNEx)
Amy J. Baldwin et al.
NUCLEIC ACIDS RESEARCH (2008)
Protein evolution with an expanded genetic code
Chang C. Liu et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2008)
Lysine acetylation can generate highly charged enzymes with increased resistance toward irreversible inactivation
Bryan F. Shaw et al.
PROTEIN SCIENCE (2008)
Site-specific insertion of 3-aminotyrosine into subunit α2 of E-coli ribonucleotide reductase:: Direct evidence for involvement of Y730 and Y731 in radical propagation
Mohammad R. Seyedsayamdost et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2007)
Developments in directed evolution for improving enzyme functions
S. Sen et al.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2007)
A genetically encoded metabolically stable analogue of phosphotyrosine in Escherichia coli
Jianming Xie et al.
ACS CHEMICAL BIOLOGY (2007)
Evolved orthogonal ribosomes enhance the efficiency of synthetic genetic code expansion
Kaihang Wang et al.
NATURE BIOTECHNOLOGY (2007)
Expanding the diversity of chemical protein modification allows post-translational mimicry
Sander I. van Kasteren et al.
NATURE (2007)
The phosphothreonine lyase activity of a bacterial type III effector family
Hongtao Li et al.
SCIENCE (2007)
Site-specific incorporation of a 19F-amino acid into proteins as an NMR probe for characterizing protein structure and reactivity
Jennifer C. Jackson et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2007)
High-throughput screening for Methionyl-tRNA synthetases that enable residue-specific incorporation of noncanonical amino acids into recombinant proteins in bacterial cells
Tae Hyeon Yoo et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2007)
An enzymatic method for site-specific labeling of recombinant proteins with oligonucleotides
Jo Tominaga et al.
CHEMICAL COMMUNICATIONS (2007)
Fluorescent protein FRET: the good, the bad and the ugly
David W. Piston et al.
TRENDS IN BIOCHEMICAL SCIENCES (2007)
Combinatorial methods for small-molecule placement in computational enzyme design
Jonathan Kyle Lassila et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2006)
A genetically encoded infrared probe
Kathryn C. Schultz et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2006)
Cation-π interaction in the polyolefin cyclization cascade uncovered by incorporating unnatural amino acids into the catalytic sites of squalene cyclase
Noriko Morikubo et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2006)
Improving nature's enzyme active site with genetically encoded unnatural amino acids
Jennifer C. Jackson et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2006)
Click chemistry as a reliable method for the high-density postsynthetic functionalization of alkyne-modified DNA
Johannes Gierlich et al.
ORGANIC LETTERS (2006)
Influence of global fluorination on chloramphenicol acetyltransferase activity and stability
Tatyana Panchenko et al.
BIOTECHNOLOGY AND BIOENGINEERING (2006)
A genetically encoded fluorescent amino acid
Jiangyun Wang et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2006)
A genetically encoded fluorescent amino acid
Daniel Summerer et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2006)
In vivo incorporation of multiple unnatural amino acids through nonsense and frameshift suppression
Erik A. Rodriguez et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2006)
A highly flexible tRNA acylation method for non-natural potypeptide synthesis
H Murakami et al.
NATURE METHODS (2006)
Insertion of an unnatural amino acid into the protein structure: preparation and properties of 3-fluorotyrosine-containing organophosphate hydrolase
Yu. A. Votchitseva et al.
RUSSIAN CHEMICAL BULLETIN (2006)
Tyrosine-selective protein alkylation using π-allylpalladium complexes
SD Tilley et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2006)
Evolving proteins of novel composition
Jin Kim Montclare et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2006)
A genetically encoded photocaged tyrosine
A Deiters et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2006)
Adding amino acids to the genetic repertoire
JM Xie et al.
CURRENT OPINION IN CHEMICAL BIOLOGY (2005)
Some special features of glyoxyl supports to immobilize proteins
C Mateo et al.
ENZYME AND MICROBIAL TECHNOLOGY (2005)
Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
RA Chica et al.
CURRENT OPINION IN BIOTECHNOLOGY (2005)
Directed evolution of enzyme stability
VGH Eijsink et al.
BIOMOLECULAR ENGINEERING (2005)
Dual-surface modification of the tobacco mosaic virus
TL Schlick et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2005)
Site-specific in vivo labeling of proteins for NMR studies
A Deiters et al.
CHEMBIOCHEM (2005)
Interior surface modification of bacteriophage MS2
JM Hooker et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2004)
Correlation between sequence hydrophobicity and surface-exposure pattern of database proteins
S Moelbert et al.
PROTEIN SCIENCE (2004)
Protein NMR in biomedical research
W Jahnke et al.
CELLULAR AND MOLECULAR LIFE SCIENCES (2004)
Structural basis for substrate selection by T7 RNA polymerase
D Temiakov et al.
CELL (2004)
A three-component Mannich-type reaction for selective tyrosine bioconjugation
NS Joshi et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2004)
A strain-promoted [3+2] azide-alkyne cycloaddition for covalent modification of blomolecules in living systems
NJ Agard et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2004)
A genetically encoded photocaged amino acid
N Wu et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2004)
Catalytic promiscuity in biocatalysis: Using old enzymes to form new bonds and follow new pathways
UT Bornscheuer et al.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2004)
Efforts towards the design of 'Teflon' proteins: In vivo translation with trifluorinated leucine and methionine analogues
N Budisa et al.
CHEMISTRY & BIODIVERSITY (2004)
Clinical development of PEGylated recombinant staphylokinase (PEG-Sak) for bolus thrombolytic treatment of patients with acute myocardial infarction
RW Moreadith et al.
ADVANCED DRUG DELIVERY REVIEWS (2003)
Global incorporation of norleucine in place of methionine in cytochrome P450 BM-3 heme domain increases peroxygenase activity
PC Cirino et al.
BIOTECHNOLOGY AND BIOENGINEERING (2003)
Enzyme stabilization -: recent experimental progress
C O'Fágáin
ENZYME AND MICROBIAL TECHNOLOGY (2003)
Chemical modification of biocatalysts
BG Davis
CURRENT OPINION IN BIOTECHNOLOGY (2003)
Reactivity of functional groups on the protein surface: Development of epoxide probes for protein labeling
G Chen et al.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2003)
Effect of pegylation on pharmaceuticals
JM Harris et al.
NATURE REVIEWS DRUG DISCOVERY (2003)
Directed evolution of enzymes: new biocatalysts for asymmetric synthesis
M Alexeeva et al.
ORGANIC & BIOMOLECULAR CHEMISTRY (2003)
Histidine and lysine as targets of oxidative modification
K Uchida
AMINO ACIDS (2003)
Incorporation of non-natural amino acids into proteins
T Hohsaka et al.
CURRENT OPINION IN CHEMICAL BIOLOGY (2002)
Fluorinated amino acids in protein design and engineering
NC Yoder et al.
CHEMICAL SOCIETY REVIEWS (2002)
Increased thermostability and phenol removal efficiency by chemical modified horseradish peroxidase
JZ Liu et al.
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2002)
Attenuation of the editing activity of the Escherichia coli Leucyl-tRNA synthetase allows incorporation of novel amino acids into proteins in vivo
Y Tang et al.
BIOCHEMISTRY (2002)
Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli
JW Chin et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2002)
Proteomic profiling of mechanistically distinct enzyme classes using a common chemotype
GC Adam et al.
NATURE BIOTECHNOLOGY (2002)
Modulation of the enantioselectivity of Candida antarctica B lipase via conformational engineering:: kinetic resolution of (±)-α-hydroxy-phenylacetic acid derivatives
JM Palomo et al.
TETRAHEDRON-ASYMMETRY (2002)
Pyrrolysine encoded by UAG in Archaea: Charging of a UAG-decoding specialized tRNA
G Srinivasan et al.
SCIENCE (2002)
De novo design of biocatalysts
DN Bolon et al.
CURRENT OPINION IN CHEMICAL BIOLOGY (2002)
Enzyme-like proteins by computational design
DN Bolon et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2001)
Elucidation of solvent exposure, side-chain reactivity, and steric demands of the trifluoromethionine residue in a recombinant protein
HS Duewel et al.
BIOCHEMISTRY (2001)
Generation of new enzymes via covalent modification of existing proteins
DF Qi et al.
CHEMICAL REVIEWS (2001)
Differential effects of isomeric incorporation of fluorophenylalanines into PvuII endonuclease
MA Dominguez et al.
PROTEINS-STRUCTURE FUNCTION AND GENETICS (2001)
Identification of an expanded set of translationally active methionine analogues in Escherichia coli
KL Kiick et al.
FEBS LETTERS (2001)
Purification and characterization of a novel phosphorus-oxidizing enzyme from Pseudomonas stutzeri WM88
AMG Costas et al.
JOURNAL OF BIOLOGICAL CHEMISTRY (2001)
Comparing the effect on protein stability of methionine oxidation versus mutagenesis: steps toward engineering oxidative resistance in proteins
YH Kim et al.
PROTEIN ENGINEERING (2001)
Expanding the genetic code of Escherichia coli
L Wang et al.
SCIENCE (2001)
Improved biocatalysts by directed evolution and rational protein design
UT Bornscheuer et al.
CURRENT OPINION IN CHEMICAL BIOLOGY (2001)
Stabilization of coiled-coil peptide domains by introduction of trifluoroleucine
Y Tang et al.
BIOCHEMISTRY (2001)
Expanding the utility of proteases in synthesis: broadening the substrate acceptance in non-coded amide bond formation using chemically modified mutants of subtilisin
K Khumtaveeporn et al.
TETRAHEDRON-ASYMMETRY (2001)
Directed evolution of enzymes for biocatalytic applications
UT Bornscheuer
BIOCATALYSIS AND BIOTRANSFORMATION (2001)
Trehalose synthesis by sequential reactions of recombinant maltooligosyltrehalose synthase and maltooligosyltrehalose trehalohydrolase from Brevibacterium helvolum
YH Kim et al.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2000)
Efficient introduction of aryl bromide functionality into proteins in vivo
N Sharma et al.
FEBS LETTERS (2000)
Pharmacokinetic and thrombolytic properties of cysteine-linked polyethylene glycol derivatives of staphylokinase
S Vanwetswinkel et al.
BLOOD (2000)
Codon usage tabulated from international DNA sequence databases: status for the year 2000
Y Nakamura et al.
NUCLEIC ACIDS RESEARCH (2000)
Selective targeting of lysosomal cysteine proteases with radiolabeled electrophilic substrate analogs
M Bogyo et al.
CHEMISTRY & BIOLOGY (2000)
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