Latest Trends on the Future of Three-Dimensional Separations in Chromatography

Separation and characterization of complex mixtures are of crucial importance in many fields, where extremely high separation power is required. Three-dimensional separation techniques can offer a path toward achieving high peak capacities. In this Review, online three-dimensional separation systems are discussed, including three-dimensional gas chromatography, and hyphenated combinations of two-dimensional gas chromatography with liquid chromatography or supercritical-fluid chromatography. Online comprehensive two-dimensional liquid chromatography provides detailed information on complex samples and the need for higher peak capacities is pushing researchers toward online three-dimensional liquid chromatography. In this review, an overview of the various combinations are provided and we discuss and compare their potential performance, advantages, perspectives, and results obtained during the most recent 10-15 years. Finally, the Review will discuss a novel approach of spatial three-dimensional liquid separation to increase peak capacity.

Automated summary

This review focuses on the importance of separation and characterization of complex mixtures, particularly in the context of online three-dimensional separation systems. Various combinations are discussed to provide detailed information on complex samples and the need for higher peak capacities. Overall, the review highlights the potential performance, advantages, perspectives, and results obtained from these systems in the past decade.