期刊
CHEMICAL ENGINEERING JOURNAL
卷 409, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2020.128131
关键词
Myocardial infarction; Blood plasma separation; Centrifuge; Paper-based microfluidics
资金
- Ministry of Science and Technology, Taiwan [109-2223-E-002-004-MY3, 108-2221-E-002-050-MY3, 108-2923-E-002-004-MY2]
- Chang Gung Memorial Hospital, Linkou [CMRPG3J0652]
- Higher Education Sprout Program at National Taiwan University, Taiwan
The study introduces a manual and portable centrifuge and a paper analytical device for plasma separation and troponin I detection, showing potential to enhance early myocardial infarction diagnosis in resource-limited regions.
Myocardial infarction (MI) causes hundreds of thousands of deaths annually. Early diagnosis of MI by detecting the concentration of troponin I in plasma can help limit mortality rates. However, plasma separation and early diagnosis are particularly difficult to achieve in resource-limited settings. In this study, we demonstrate a manual and portable centrifuge along with a paper analytical device to measure troponin I for early MI diagnosis. With this so-called Handfuge, pure plasma can be achieved with a yield of up to 47.1% and purity as high as 99.9% in less than 3 min without electricity. The resulting plasma can then be directly used for detecting tmponin I using a paper-based enzyme-linked immunosorbent assay (ELISA), with a detection limit as low as 0.03 ng/mL. The whole detection process consumes just 10 mu L. of blood, which is similar to the volume obtained by a clinical finger-prick. The proposed centrifuge together with the paper analytical device possesses the potential to replace existing techniques in tmponin I detection for early MI diagnoses in resource-limited regions of the world.
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