期刊
CELLULAR MICROBIOLOGY
卷 23, 期 8, 页码 -出版社
WILEY
DOI: 10.1111/cmi.13340
关键词
adhesin antibody; Burkholderia cepacia complex; host glycans as adhesin targets; trimeric autotransporter adhesins
资金
- Fundacao para a Ciencia e a Tecnologia [PD/BD/116940/2016, UID/BIO/04565/2020]
- Programa Operacional Regional de Lisboa 2020 [007317]
- Fundação para a Ciência e a Tecnologia [PD/BD/116940/2016] Funding Source: FCT
The study revealed the adhesive role of TAA in Burkholderia and the type of host glycan structures they target, showing that neutralization of BCAM2418 reduces bacterial adhesion and that anti-BCAM2418 antibody has inhibitory effects in vivo. The study also identified host glycan epitopes for a TAA for the first time and demonstrated similar specificities of Burkholderia species for their most intensely binding carbohydrates through glycan microarrays.
Trimeric Autotransporter Adhesins (TAA) found in Gram-negative bacteria play a key role in virulence. This is the case of Burkholderia cepacia complex (Bcc), a group of related bacteria able to cause infections in patients with cystic fibrosis. These bacteria use TAAs, among other virulence factors, to bind to host protein receptors and their carbohydrate ligands. Blocking such contacts is an attractive approach to inhibit Bcc infections. In this study, using an antibody produced against the TAA BCAM2418 from the epidemic strain Burkholderia cenocepacia K56-2, we were able to uncover its roles as an adhesin and the type of host glycan structures that serve as recognition targets. The neutralisation of BCAM2418 was found to cause a reduction in the adhesion of the bacteria to bronchial cells and mucins. Moreover, in vivo studies have shown that the anti-BCAM2418 antibody exerted an inhibitory effect during infection in Galleria mellonella. Finally, inferred by glycan arrays, we were able to predict for the first time, host glycan epitopes for a TAA. We show that BCAM2418 favoured binding to 3 ' sialyl-3-fucosyllactose, histo-blood group A, alpha-(1,2)-linked Fuc-containing structures, Lewis structures and GM1 gangliosides. In addition, the glycan microarrays demonstrated similar specificities of Burkholderia species for their most intensely binding carbohydrates.
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