期刊
CELLULAR AND MOLECULAR BIOENGINEERING
卷 14, 期 4, 页码 349-363出版社
SPRINGER
DOI: 10.1007/s12195-021-00671-2
关键词
Tribological rehydration; Fluid load support; Osteoarthritis; Cartilage tribology; Convergent stationary contact area; Path analysis
资金
- National Science Foundation (NSF) Biomaterials and Mechanobiology program [1635536]
- NSF Graduate Research Fellowship Program [1247394]
- Div Of Civil, Mechanical, & Manufact Inn
- Directorate For Engineering [1635536] Funding Source: National Science Foundation
Research has shown that the survival of chondrocytes is impacted by tribological rehydration, with the speed of friction affecting the rate of cell death. Testing on compressed equine cartilage explants revealed that different friction speeds directly impacted cell death and physiological environments.
In vivo, articular cartilage is exceptionally resistant to wear, damage, and dysfunction. However, replicating cartilage's phenomenal in vivo tribomechanics (i.e., high fluid load support, low frictions and strains) and mechanobiology on the benchtop has been difficult, because classical testing approaches tend to minimize hydrodynamic contributors to tissue function. Our convergent stationary contact area (cSCA) configuration retains the ability for hydrodynamically-mediated processes to contribute to interstitial hydration recovery and tribomechanical function via 'tribological rehydration'. Using the cSCA, we investigated how in situ chondrocyte survival is impacted by the presence of tribological rehydration during the reciprocal sliding of a glass counterface against a compressively loaded equine cSCA cartilage explant. When tribological rehydration was compromised during testing, by slow-speed sliding, 'pathophysiological' tribomechanical environments and high surface cell death were observed. When tribological rehydration was preserved, by high-speed sliding, 'semi-physiological' sliding environments and suppressed cell death were realized. Inclusion of synovial fluid during testing fostered 'truly physiological' sliding outcomes consistent with the in vivo environment but had limited influence on cell death compared to high-speed sliding in PBS. Subsequently, path analysis identified friction as a primary driver of cell death, with strain an indirect driver, supporting the contention that articulation mediated rehydration can benefit both the biomechanical properties and biological homeostasis of cartilage.
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