期刊
CELLS TISSUES ORGANS
卷 211, 期 3, 页码 313-323出版社
KARGER
DOI: 10.1159/000513118
关键词
Borrelia burgdorferi; Complement; Lyme disease; Microfluidic; Neutrophil; Oscillation; Phagocytosis; Positive feed-back; Spontaneous migration
资金
- National Institutes of Health [GM092804, EB002503]
Human neutrophils in blood samples from both Lyme disease patients and healthy donors exhibit robust migratory responses to Borrelia burgdorferi, which are complement-dependent. Inhibition of complement factor 5 cleavage reduces neutrophil-Bb interactions and spontaneous neutrophil motility. The microfluidic assay is validated as a useful tool for studying neutrophil-Bb interactions in blood samples.
Human neutrophils are highly sensitive to the presence of Borrelia burgdorferi (Bb), the agent of Lyme disease (LD), in tissues. Although Bb is also found in the blood of LD patients, far less is known about how neutrophils respond to Bb in the presence of blood. In this study, we employed microfluidic tools to probe the interaction between human neutrophils and Bb and measured the activation of human neutrophils in blood samples from patients. We found that neutrophils migrate vigorously toward Bb in the presence of serum, and this process was complement-dependent. Preventing complement factor 5 cleavage or blocking complement receptors decreased neutrophil's ability to interact with Bb. We also found that spiking Bb directly into the blood from healthy donors induced spontaneous neutrophil motility. This response to Bb was also complement-dependent. Preventing complement factor 5 cleavage decreased spontaneous neutrophil motility in Bb-spiked blood. Moreover, we found that neutrophils in blood samples from acute LD patients displayed spontaneous motility patterns similar to those observed in Bb-spiked samples. Neutrophil motility was more robust in blood samples from LD patients than that measured in healthy and ill controls, validating the utility of the microfluidic assay for the study of neutrophil-Bb interactions in the presence of blood.
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