期刊
CELL STRESS & CHAPERONES
卷 26, 期 4, 页码 629-637出版社
SPRINGER
DOI: 10.1007/s12192-021-01205-5
关键词
β -Glucan; Oxidative stress; Dectin-1; Nrf2; HO-1
类别
资金
- National Natural Science Foundation of China [32060774]
- China Agriculture Research System [CARS-36]
- Inner Mongolia Autonomous Region Science and Technology Plan Project [2020GG0036]
Beta-glucan from Saccharomyces cerevisiae has been shown to alleviate oxidative stress in LPS-stimulated RAW264.7 cells by activating the Dectin-1/Nrf2/HO-1 signaling pathway, leading to decreased ROS production.
beta-Glucan from Saccharomyces cerevisiae has been described to be effective antioxidants, but the specific antioxidation mechanism of beta-glucan is unclear. The objectives of this research were to determine whether the beta-glucan from Saccharomyces cerevisiae could regulate oxidative stress through the Dectin-1/Nrf2/HO-1 signaling pathway in lipopolysaccharides (LPS)-stimulated RAW264.7 cells. In this study, we examined the effects of beta-glucan on the enzyme activity or production of oxidative stress indicators in LPS-stimulated RAW264.7 cells by biochemical analysis and the protein expression of key factors of Dectin-1/Nrf2/HO-1 signaling pathway by immunofluorescence and western blot. The biochemical analysis results showed that beta-glucan increased the LPS-induced downregulation of enzyme activity of intracellular heme oxygenase (HO), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) while decreasing the production of reactive oxygen species (ROS) and malondialdehyde (MDA). Furthermore, immunofluorescence results showed that beta-glucan can activate the nuclear factor erythroid 2-related factor 2 (Nrf2). The antioxidant mechanism study indicated that beta-glucan activated dendritic-cell-associated C-type lectin 1 (Dectin-1) receptors mediated Nrf2/HO-1 signaling pathway, thereby downregulating the production of ROS and thus produced the antioxidant effects in LPS-stimulated RAW 264.7 cells. In conclusion, these results indicate that beta-glucan potently alleviated oxidative stress via Dectin-1/Nrf2/HO-1 in LPS-stimulated RAW 264.7 cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据