期刊
CELL AND TISSUE BANKING
卷 23, 期 1, 页码 157-170出版社
SPRINGER
DOI: 10.1007/s10561-021-09926-8
关键词
DPSC; UCSC; Replicative senescence; ROS; Biological wastes
资金
- Indian Council of Medical Research
MSCs are potential sources for tissue regeneration and cell-based therapy, and can be isolated from various body tissues and biological waste sources. The source of MSCs influences their proliferation capacity and replicative senescence, urging the need for comparative studies to select appropriate stem cell sources for regenerative medicine applications in the future.
Mesenchymal stem cells (MSC) have been widely studied for tissue regeneration and cell-based therapy. MSC can be isolated from different body tissues while several biological waste sources like dental pulp, umbilical cord, cord derived blood, amniotic fluid or urine have also emerged as potential sources of MSCs. Specifically, isolation of MSCs from such non-conventional sources show promising outcomes due to the non-invasiveness of the extraction process and high proliferation capacity of the isolated MSC. However, these stem cells also exhibit the limitation of replicative senescence in long-term culture condition. Inter-cellular reactive oxygen species is an important contributor for inducing cellular senescence under long-term culture conditions. For translational application, it becomes imperative to compare the stem cells isolated from these sources for their senescence and proliferative properties. In this study, MSC were extracted from two different sources of biological waste materials-dental pulp and umbilical cord, and compared for their proliferation capacity and replicative senescence at different passage numbers (i.e. P2 and P6). Intracellular ROS production was significantly (p < 0.001) less in dental pulp stem cells culture in comparison to umbilical cord-derived stem cells at P6. The beta-gal expression also showed significantly (p < 0.001) low expression in DPSC culture compared to that of UCSC at P6. The study indicates the source of stem cells influences the proliferation capacity as well as replicative senescence of MSCs. This study will thus pave the path of future research in selecting appropriate stem cell source for regenerative medicine application.
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