期刊
CELL
卷 184, 期 11, 页码 2878-+出版社
CELL PRESS
DOI: 10.1016/j.cell.2021.04.012
关键词
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资金
- Biowulf
- High-Performance Computing Group
- Center for Information Technology
- Intramural Research Program of the NIH
This study establishes a platform for observing the dynamics of RNA synthesis and processing at a single molecule level, revealing transcriptional bursting in all genes and significant kinetic variation in splicing within introns. The research also uncovers widespread stochastic recursive splicing within introns and proposes a unified theoretical model to explain these phenomena.
The activities of RNA polymerase and the spliceosome are responsible for the heterogeneity in the abundance and isoform composition of mRNA in human cells. However, the dynamics of these megadalton enzymatic complexes working in concert on endogenous genes have not been described. Here, we establish a quasi-genome-scale platform for observing synthesis and processing kinetics of single nascent RNA molecules in real time. We find that all observed genes show transcriptional bursting. We also observe large kinetic variation in intron removal for single introns in single cells, which is inconsistent with deterministic splice site selection. Transcriptome-wide footprinting of the U2AF complex, nascent RNA profiling, long-read sequencing, and lariat sequencing further reveal widespread stochastic recursive splicing within introns. We propose and validate a unified theoretical model to explain the general features of transcription and pervasive stochastic splice site selection.
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