期刊
CARCINOGENESIS
卷 42, 期 6, 页码 831-841出版社
OXFORD UNIV PRESS
DOI: 10.1093/carcin/bgab034
关键词
-
类别
资金
- National Natural Science Foundation of China [82073628, 81871876, 81672303, 81402753, 81872694, 81673267, 81602289, 81872127]
- Guangzhou Science and Technology Program Pearl River Nova projects [201710010049]
- National Key Research and Development Program of China [2017YFC0907100]
- Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program [2017BT01S155]
This study utilized RNA sequencing analysis to profile serum exosomal lncRNAs in NSCLC patients and pneumonia controls, identifying a promising lncRNA with diagnostic and prognostic value. High expression of exosomal linc01125 was associated with unfavorable overall survival in NSCLC, potentially inhibiting cancer growth and metastasis by up-regulating TNFAIP3 expression through sponging miR-19b-3p. Lung cancer cells were found capable of releasing linc01125 into exosomes both in vitro and in vivo, suggesting its potential as a non-invasive biomarker for diagnosing and predicting NSCLC prognosis.
A non-invasive method to distinguish potential lung cancer patients would improve lung cancer prevention. We employed the RNA-sequencing analysis to profile serum exosomal long non-coding RNAs (lncRNAs) from non-small cell lung cancer (NSCLC) patients and pneumonia controls, and then determined the diagnostic and prognostic value of a promising lncRNA in four datasets. We identified 90 dysregulated lncRNAs for NSCLC and found the most significant lncRNA was a novel isoform of linc01125. Serum exosomal linc01125 could distinguish NSCLC cases from disease-free and tuberculosis controls, with the area under the curve values as 0.662 [95% confidence interval (CI) = 0.614-0.7111 and 0.624 (95% CI = 0.522-0.725), respectively. High expression of exosomal linc01125 was also correlated with an unfavorable overall survival of NSCLC (hazard ratio = 1.48, 95% CI = 1.05-2.08). Clinic treatment decreased serum exosomal linc01125 in NSCLC patients (P = 0.036). Linc01125 functions to inhibit cancer growth and metastasis via acting as a competing endogenous RNA to up-regulate tumor necrosis factor alpha-induced protein 3 (TNFAIP3) expression by sponging miR-19b-3p. Notably, the oncogenic transformation of 16HBE led to decreased linc01125 in cells but increased linc01125 in cell-derived exosomes. The expression of linc01125 in total exosomes was highly correlated with that in tumor-associated exosomes in serum. Moreover, lung cancer cells were capable of releasing linc01125 into exosomes in vitro and in vivo. Our analyses suggest serum exosomal linc01125 as a promising biomarker for non-invasively diagnosing NSCLC and predicting the prognosis of NSCLC.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据