A long-term controlled drug-delivery with anionic beta cyclodextrin complex in layer-by-layer coating for percutaneous implants devices

This study demonstrated a drug-delivery system with anionic beta cyclodextrin (beta-CD) complexes to retain tetracycline (TC) and control its release from multilayers of poly(acrylic acid) (PAA) and poly(l-lysine) (PLL) in a ten double layers ([PAA/PLL](10)) coating onto titanium. The drug-delivery capacity of the multilayer system was proven by controlled drug release over 15 days and sustained released over 30 days. Qualitative images confirmed TC retention within the layer-by-layer (LbL) over 30 days of incubation. Antibacterial activity of TC/anionic beta-CD released from the LbL was established against Staphylococcus aureus species. Remarkably, [PAA/ PLL] 10/TC/anionic beta-CD antibacterial effect was sustained even af ter 30 days of incubation. The non-cytotoxic effect of the multilayer system revealed normal human gingival fibroblast growth. It is expected that this novel approach and the chemical concept to improve drug incorporation into the multilayer system will open up possibilities to make the drug release system more applicable to implantable percutaneous devices.

Automated summary

This study developed a drug delivery system using anionic beta cyclodextrin complexes to retain and control the release of tetracycline on titanium surface. The system showed controlled drug release over 15 days, sustained release over 30 days, and antibacterial activity against Staphylococcus aureus. The non-cytotoxic effect on human gingival fibroblast growth was also demonstrated.