期刊
CANCER SCIENCE
卷 112, 期 6, 页码 2393-2404出版社
WILEY
DOI: 10.1111/cas.14883
关键词
biomarker; diagnosis; microRNA; nasopharyngeal carcinoma; small extracellular vesicle
类别
资金
- Key Research and Development Program of Guangxi [Guike AB18281003]
- Guangxi Medical and Health Appropriate Technology Development and Promotion Application Project [S2017020]
- Middle-aged and Young Teachers' Basic Ability Promotion Project of Guangxi [2018KY0134]
- Guangxi Science and Technology Program Project [GK AD17129013]
- National Natural Science Foundation of China [82060019, 82060494]
The study evaluated circulating sEV-derived miRNAs as potential biomarkers for the diagnosis of nasopharyngeal carcinoma (NPC). It identified a distinct set of differentially expressed miRNAs between NPC patients and healthy controls, with target genes mainly involved in cancer-associated signaling pathways. A 3 miRNA-based model showed good discriminating power in diagnosing NPC and had an excellent ability to distinguish NPC patients at different stages from healthy controls.
The microRNAs (miRNAs) in circulating small extracellular vesicles (sEVs) have been suggested as potential biomarkers in cancer diagnosis. This study was designed to evaluate the circulating sEV-derived miRNAs as biomarkers for the diagnosis of nasopharyngeal carcinoma (NPC). We compared the miRNA profiles in plasma-derived sEVs between 16 patients with NPC and 5 healthy controls (HCs). A distinct set of miRNAs that were differentially expressed between patients with NPC and HCs was determined by means of integrative bioinformatics approaches. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment pathway analysis revealed that the target genes of the differentially expressed miRNAs (DEMs) were mainly involved in cancer-associated signaling pathways. Seven representative DEMs were selected and further validated in an additional 60 patients with NPC and 40 HCs using quantitative reverse-transcription PCR analysis (qRT-PCR). Receiver operating characteristic (ROC) curve analysis was used to assess the accuracy of the sEV-miRNA-based model for diagnosis. The 3 miRNA-based model, comprising miR-134-5p, miR-205-5p, and miR-409-3p, showed good discriminating power with an area under the curve (AUC) value of 0.88 in the training set and 0.91 in the validation set. Furthermore, the diagnostic model had an excellent classification ability to distinguish patients with NPC at different clinical stages or Epstein-Barr virus infection status from HCs. In conclusion, our findings indicated that sEV-derived miRNA levels were altered in the plasma of patients with NPC in comparison with those in HCs. The model based on the 3 sEV-derived miRNAs could potentially act as an alternative or complementary approach for diagnosing NPC.
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