期刊
CANCER CELL INTERNATIONAL
卷 21, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12935-021-01871-6
关键词
Hepatocellular carcinoma; WGCNA; SLC27A5; Migration; Epithelial-mesenchymal transition
类别
资金
- National Key Research and Development Program of China [2018YFC2002000]
- National Natural Science Foundation of China [81901408]
- Shanghai Sailing program [19YF1414500]
- Shanghai Medical Leadership Training Program [2019LJ09]
This study identified a hub module associated with hepatocellular carcinoma (HCC) prognosis through co-expression network analysis, with SLC27A5 identified as a potential prognostic marker. Experimental evidence showed that downregulation of SLC27A5 promoted the progression of HCC.
BackgroundThe incidence and mortality rates of hepatocellular carcinoma are among the highest of all cancers all over the world. However the survival rates are relatively low due to lack of effective treatments. Efforts to elucidate the mechanisms of HCC and to find novel prognostic markers and therapeutic targets are ongoing. Here we tried to identify prognostic genes of HCC through co-expression network analysis.MethodsWe conducted weighted gene co-expression network analysis with a microarray dataset GSE14520 of HCC from Gene Expression Omnibus database and identified a hub module associated with HCC prognosis. Function enrichment analysis of the hub module was performed. Clinical information was analyzed to select candidate hub genes. The expression profiles and survival analysis of the selected genes were performed using additional datasets (GSE45267 and TCGA-LIHC) and the hub gene was identified. GSEA and in vitro experiments were conducted to further verify the function of the hub gene.ResultsGenes in the hub module were mostly involved in the metabolism pathway. Four genes (SLC27A5, SLC10A1, PCK2 and FMO4) from the module were identified as candidate hub genes according to correlation analysis with prognostic indicators. All these genes were significantly down-regulated in tumor tissues compared with non-tumor tissues in additional datasets. After survival analysis and network construction, SLC27A5 was selected as a prognostic marker. GSEA analysis and in vitro assays suggested that SLC27A5 downregulation promoted tumor cell migration via enhancing epithelial-mesenchymal transition.ConclusionSLC27A5 is a potential biomarker of HCC and SLC27A5 downregulation promoted HCC progression by enhancing EMT.
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