Draft genome sequence of Lactobacillus delbrueckii subsp. bulgaricus LBP UFSC 2230: a tool for preliminary identification of enzymes involved in CLA metabolism

Several Lactobacillus ssp. are recognized as potential conjugated linoleic acid (CLA) producers. We have previously reported the ability of a range of Lactobacillus delbrueckii subsp. bulgaricus strains to produce CLA in fermented milk, being a potential candidate for the fermented dairy food chain. This study reports the draft genome sequence of L. bulgaricus strain LBP UFSC 2230, isolated from Italian Grana Padano cheese. Draft genome sequence originated in a total of 4,310,842 paired-end reads that were quality trimmed and assembled into 135 contigs with a total length of 604,745,873 bp, including 2086 protein coding genes and an average GC content of 49.7%. Draft genome sequence represents an important tool to identify the enzymes involved in this strain's CLA metabolism. We identified a gene encoding an enzyme involved in biohydrogenation of linoleic acid pathway, oleate hydratase.

Automated summary

Several strains of Lactobacillus are known as potential producers of conjugated linoleic acid (CLA), with L. bulgaricus strain LBP UFSC 2230 being identified as a candidate for CLA production in fermented dairy products. The draft genome sequence of this strain, isolated from Italian Grana Padano cheese, provides valuable insights into its metabolic capabilities, particularly the identification of an enzyme involved in linoleic acid biohydrogenation, oleate hydratase.