期刊
BRAIN RESEARCH BULLETIN
卷 170, 期 -, 页码 199-210出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.brainresbull.2021.02.015
关键词
Spinal cord injury; microRNA-125a; IRF5; Bone marrow mesenchymal stem cells-derived exosomes; Macrophage M2
B-Exo promotes recovery of locomotor function and M2-phenotype polarization, inhibits neuronal apoptosis and degeneration, as well as the inflammatory response in rats with SCI. IRF5 expression is reduced after B-Exo treatment, and it promotes macrophage polarization towards M1 phenotype and secretion of inflammatory factors. There is a binding relationship between miR-125a and IRF5, with the knockdown of miR-125a in B-Exo leading to increased IRF5 expression and attenuation of the neuroprotective effect against SCI.
Background: Spinal cord injury (SCI) may cause loss of locomotor function, and macrophage is a major cell type in response to SCI with Ml- and M2-phenotypes. The protective role of bone marrow mesenchymal stem cells (BMMSC)-derived exosomes (B-Exo) in SCI has been underscored, while their regulation on M2 macrophage polarization and the mechanism remain to be clarified. Methods: A rat model of SCI was developed and treated with extracted B-Exo. Recovery of motor function was assessed by Basso-Beattie-Bresnahan (BBB) score. The apoptosis and degeneration of neurons, and macrophage polarization were evaluated. Subsequently, genes differentially expressed in the rat spinal cord after B-Exo treatment were analyzed. Later, the relationships between B-Exo and interferon regulatory factor 5 (IRF5) or macrophage polarization were clarified. Later, the upstream microRNAs (miRNAs) of IRF5 were validated by bioinformatics prediction and dual-luciferase experiments. Finally, the role of miR-125a in the neumprotection of SCI was verified by rescue experiments. Results: B-Exo promoted the recovery of locomotor function and M2-phenotype polarization, whereas inhibited neuronal apoptosis and degeneration and the inflammatory response caused by SCI in rats. In addition, IRF5 expression was reduced after B-Exo treatment. IRF5 promoted macrophage polarization towards Ml-phenotype and secretion of inflammatory factors. There is a binding relationship between miR-125a and IRF5. Knockdown of miR-125a in B-Exo increased IRF5 expression in spinal cord tissues of SCI rats and attenuated the neuroprotective effect of B-Exo against SCI. Conclusion: Exosomal miR-125a derived from BMMSC exerts neuroprotective effects by targeting and negatively regulating IRF5 expression in SCI rats.
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