期刊
BRAIN RESEARCH BULLETIN
卷 173, 期 -, 页码 141-149出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.brainresbull.2021.05.016
关键词
Human pluripotent stem cells; Neural differentiation; Culture media; Glucose; Insulin
资金
- Melbourne Research Scholarship (MRS)
- Murdoch Children's Research Institute, The University of Melbourne
- Australian Research Council
- Stem Cells Australia
A new hPSC neural differentiation medium with physiological concentrations of glucose and reduced insulin levels was established in this study, supporting hPSC neural induction and formation of cortical NSPs. This revised medium may offer an improved platform for studying brain development and investigating neural differentiation signalling pathways impacted by abnormal glucose and insulin levels.
Cortical neurospheres (NSPs) derived from human pluripotent stem cells (hPSC), have proven to be a successful platform to investigate human brain development and neuro-related diseases. Currently, many of the standard hPSC neural differentiation media, use concentrations of glucose (approximately 17.5-25 mM) and insulin (approximately 3.2 mu M) that are much greater than the physiological concentrations found in the human brain. These culture conditions make it difficult to analyse perturbations of glucose or insulin on neuronal development and differentiation. We established a new hPSC neural differentiation medium that incorporated physiological brain concentrations of glucose (2.5 mM) and significantly reduced insulin levels (0.86 mu M). This medium supported hPSC neural induction and formation of cortical NSPs. The revised hPSC neural differentiation medium, may provide an improved platform to model brain development and to investigate neural differentiation signalling pathways impacted by abnormal glucose and insulin levels.
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